Abstract
Summary: Chemotactic mobility of cord blood granulocytes (CBG) was studied under varying concentrations of synthesized chemotactic peptide, N-formyl-methionyl-leucyl-phenylalanine(f · Met-Leu-Phe). The maximal chemotactic mobility was found at a concentration of 2 X 10-7 M in CBG and 1 X 10-7M in adult blood granulocytes (ABG) (n = 8). The maximal distance of granulocyte mobility of CBG was significantly shorter than that of ABG (P < 0.01). The number of chemotactic receptors and the affinity constant were assayed by the competitive inhibition method, using f · Met-Leu-|3H|Phe, and the data were subjected to Scatchard analysis. The number of chemotactic receptors of ABG was 2.5-fold of CBG, as shown by 21,800 ± 7800 per cell in ABG (n = 3) and 9000 fat CBG (n = 1). This figure was confirmed by the one point assay method by increasing the sample numbers of cord blood. It was found that bound chemotactic peptide (x 10-14 moles/107 cells) was 7.9 ± 0.7 in ABG (n = 4) and 3.4 ± 0.7 in CBG (n = 5). Affinity constants were similar in CBG and ABG.
Speculation: Reduced chemotactic mobility in cord blood granulocytes is attributable to the decreased number of chemotactic receptors and the disturbed association between cell surface receptors and intracellular metabolism.
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Nunoi, H., Endo, F., Chikazawa, S. et al. Chemotactic Receptor of Cord Blood Granulocytes to the Synthesized Chemotactic Peptide N-Formyl-Methionyl-Leucyl-Phenylalanine. Pediatr Res 17, 57–60 (1983). https://doi.org/10.1203/00006450-198301000-00011
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DOI: https://doi.org/10.1203/00006450-198301000-00011