Abstract
We have reported on Leukocyte Migration Inhibition(LMI) as a reliable in vitro assay of antigen-specific activity in human leukocyte dialysates (DLE) containing Transfer Factor. We have shown that treatment of nonimmune leukocytes with specific DLE renders them immune to the antigen and DLE treatment of immune leukocytes renders them nonimmune to the antigens to which the DLE donor was sensitive. These properties are designated “Inducer DIE” (I-DLE) and “Suppressor DLE” (S-DLE) respectively (J.I.4/79). Specific activity of I-DLE binds to specific antigen-immunoabsorbent but not to specific antibody-immunoabsorbent (J.I.2/81). We now suggest that I-DLE AND S-DLE are separate moieties since 1) the antigen specific activity in I-DLE binds to an affinity purified anti-VH antibody but not to anti-VL chain antibody(prepared by Givol) while S-DLE binds to the anti-VL chain antibody; & 2) that S-DLE does not bind antigen; preliminary results suggest that it binds IgG. This suggests that antigen specific activity in I-DLE may represent a dialysable fragment of antigen binding sites of immune T-cells; I-DLE and S-DLE may be representative of a network system.
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Borkowsky, W., Lawrence, H. 901 ANTIGEN-SPECIFIC INDUCER ACTIVITY IN HUMAN LEUKOCYTE DIAI YSATES BINDS TO ANTI-VH BUT NOT ANTI-VL CHAIN ANTIBODY WHILE ANTIGEN SPECIFIC SUPPRESSOR ACTIVITY BINDS TO ANTI-VL CHAIN ANTIBODY. Pediatr Res 15 (Suppl 4), 592 (1981). https://doi.org/10.1203/00006450-198104001-00926
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DOI: https://doi.org/10.1203/00006450-198104001-00926