Abstract
Recently, normal and modified urinary nucleosides and nucleobases have been shown to reflect processes of proliferation, differentiation, and malignant transformation1. To realize the theoretical implications of those findings and ideas for clinical applications it was necessary to develop a highly sensitive, rapid, and reliable analytical procedure. Several prefractionation steps were combined to isolate bulk nucleosides and nucleobases simultaneously. Finally, both were analysed by reversed phase HPLC (detection limit: 10-13 mole per substance; about 1ml of urine, serum or spinal fluid is sufficient for a complete analysis). The registration and comparison of the analytical data has been automated on the basis of creatinine-, age- and sex-correlated regression equations of normal values. Actually, 17 normal and modified nucleosides and nucleobases are routinely measured. We use this method presently to control effects of cytostatic therapy as well as normal growth velocity.
Schoech, G. et al. Helv.Paediat.Acta, Suppl. 38, 1-171, 1977.
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Schoech, G., Thomale, J. & Schäfer, K. A new method for the rapid analysis of normal and modified nucleosides and nucleobases in various “body fluids: 50. Pediatr Res 14, 173 (1980). https://doi.org/10.1203/00006450-198002000-00077
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DOI: https://doi.org/10.1203/00006450-198002000-00077