Abstract
The effect of isoproterenol hydrochloride (I) on peripheral blood lymphocytes (PBL) rosette formation with sheep red blood cells (E) coated with C3 (EAC3) was studied. PBL from 25 allergic asthmatics, 25 normal subjects and 10 subjects with allergic rhinitis were separated over density gradients and incubated in RPMI medium and fetal calf serum (FCS) at 37°C overnight. From each subject 0.8 × 106 PBL were incubated with phosphate buffered saline or I for one hour at 37°C, washed 3 times in Hanks1 medium with FCS and rosetted with both E and EAC3 reagent.
E rosette formation was similar for all groups and unaffected by I. Baseline EAC3 rosette number was similar for all groups (normals:12%; rhinitis:13%; asthma:13%). Incubation with I of PBL from normal subjects and patients with rhinitis resulted in statistically significant increases in the number of EAC3 rosette forming cells (normals-base:12%, I:19%, p<.001; rhinitis-base:13% I:20%, p<.001). Incubation with I of PBL from asthmatics resulted in no increase in the number of EAC3 rosette forming cells (asthma-base:13%, I:13%). Similar results were obtained with theophylline (Th) in 15 pairs of normals and asthmatics. Bronchodilator therapy did not affect the results in either normals (3) or asthmatics. The resistance of PBL from asthmatics to the enhancing effect of I and Th on EAC3 rosette formation may be used to study the biochemical defect in asthma and may provide an in vitro test for the detection of the latent asthmatic.
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Kohn, B., Geha, R. 708 RESISTANCE OF BLOOD LYMPHOCYTES IN ASTHMA TO THE ENHANCING EFFECT OF ISOPROTERENOL ON ROSETTE FORMATION WITH EAC1423 (EAC3). Pediatr Res 12 (Suppl 4), 481 (1978). https://doi.org/10.1203/00006450-197804001-00713
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DOI: https://doi.org/10.1203/00006450-197804001-00713