Abstract
The role of eosinophiles in hematopoiesis is not clear. Despite the presence of eosinophilia in a variety of parasitic and other conditions, the leukocyte counts frequently are not elevated. This suggests a possible inhibitory effect of eosinophiles on granulopoiesis. This study was undertaken to examine the effects of eosinophiles on granulocytic colony forming units in culture (CFU-C) of mice bone marrow (BM). Marrow of normal as well as eosinophilic inbread C-57 black mice infected with Trichinella spiralis was cultured in semi-solid media with 1) anti-eosinophilic sera produced against mice eosinophiles in rabbit (AES), 2) the addition of purified eosinophiles, 3) colony stimulating activity (CSA), 4) α-culture media, 5) normal rabbit serum, 6) eosinophilia extracts, and 7) irradiated eosinophiles. Each of the above experiments was repeated with and without added CSA. Addition of AES to BM cultures containing CSA sharply increased CFU-C levels in eosinophilic mice compared to CSA and rabbit serum (controls) alone (P<0.01), but had no effect on normal BM. Addition of increased numbers of eosinophiles significantly decreased the number of CFU-C in normal and eosinophilic mice (P<0.01). CSA was necessary to promote colony production and to enhance the effects of AES in eosinophilic mice. The results of this experiment suggest that eosinophiles have a suppressive effect on production of granulopoietic colonies and may indicate an inhibitory effect of these cells on total granulopoietic activity. This inhibitory effect of eosinophiles may be due to their high content of Protoglandin E.
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Tebbi, K., Mahmoud, A. & Gross, S. 670 THE ROLE OF EOSINOPHILES IN GRANULOPOIESIS. Pediatr Res 12 (Suppl 4), 475 (1978). https://doi.org/10.1203/00006450-197804001-00675
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DOI: https://doi.org/10.1203/00006450-197804001-00675