Abstract
Infected urine specimens (IU) collected during 13 episodes of UTI (colony count>105/ml) from girls (mean age 5 1/2 years) and an equal number of sterile normal urine (NU) specimens from uninfected subjects were studied for chemotactic properties. Diagnosis of upper UTI was based on positive antibody coated bacteria (ACB) test. Chemotaxis was performed by modified Boyden's technique utilizing PMNS from healthy volunteers. Cells placed on 3 micropore filter in the upper chamber were simultaneously tested against 1) Hanks' solution (HS). 2) HS containing 25, 50 and 100μl/ml of urinary supernatant filtrates. 3) Endo-toxin activated normal serum (EAS) placed in the lower chamber. The mean (±1SD) chemotactic indices (CI) are presented in the table. IU in all concentrations were more chemotactic than NU
and HS (P <0.005). ACB + IU showed higher CIs (P< 0.005) than ACB negative IU in each concentration. CIs with 100μl/ml of ACB + IU were comparable to CIs obtained with EAS. PMNS chemotaction may aid in diagnosis of UTI and in differentiation of upper from lower UTI. It may be speculated that higher CIs in upper UTI may be due to unknown chemotactic factor(s) elaborated by renal tissue in addition to those produced by the organisms.
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Khan, A., Kumar, K., Khan, P. et al. 1081 NEUTROPHIL (PMNS) CHEMOTACTION BY URINE CORRELATED WITH UPPER URINARY TRACT INFECTION (UTI). Pediatr Res 12 (Suppl 4), 544 (1978). https://doi.org/10.1203/00006450-197804001-01087
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DOI: https://doi.org/10.1203/00006450-197804001-01087