Abstract
Ninety per cent of H. influenzae from middle ear effusions are non-typable (HiNT). We have used a bactericidal antibody assay to classify HiNT and detect specific antibody. Rabbit antisera to two strains (T1 and T2), with complement, kill homologous organisms. Anti-T2 is specific after absorption with T1 bacteria(anti-T2 abs). Sensitivity to these sera distinguished 4 serogroups among 22 HiNT strains from middle ear effusion:
Human sera were tested for activity against T1, T2, an SR strain, and a type b strain:
The age and strain differences in prevalence of antibody demonstrate the specificity of this typing scheme. Classification of HiNT should facilitate epidemiologic study of otitis media.
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Shurin, P., Pelton, S., Tager, I. et al. BACTERICIDAL ANTIBODY TO NON-TYPABLE STRAINS OF HEMOPHILUS INFLUENZAE. Pediatr Res 11, 505 (1977). https://doi.org/10.1203/00006450-197704000-00814
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DOI: https://doi.org/10.1203/00006450-197704000-00814