Abstract
We have previously shown that CMI to RSV is common in adults and absent in susceptible neonates. To further define imminologic responses to RSV, we studied 24 children hospitalized from 7-12/76 and 34 healthy adults. Heparinized whole blood was diluted with RPMI-1640 and incubated for 6 d. with antigens prepared from RSV infected Vero cells, from uninfected control cells, with no antigen and with phytohemagglutinin (PHA). Triplicate cultures were labelled with 3H-thymidine. Stimulation indices (SI = mean DPM RSV culture/mean DPM control culture) were positive if over 2.0 and if the means were significantly different. Ten children had respiratory illnesses. Nasopharyngeal cultures for RSV were negative. Lymphocyte reactivity occurred as shown:
Children were intermediate between infants and adults in frequency of reactivity to RSV antigens. The higher frequency of reactivity in adults may result from more RSV exposures. The absence of reactivity to RSV in 9 infants under age 1 yr agrees with our previous observations in neonates. We speculate that lack of CMI may be associated with the susceptibility of this group to lower respiratory tract infection by RSV.
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Purcell, C., Mizen, M., Schauf, V. et al. CELL-MEDIATED IMMUNITY (CMI) TO RESPIRATORY SVNCYTIAL VIRUS (RSV) IN CHILDREN. Pediatr Res 11, 492 (1977). https://doi.org/10.1203/00006450-197704000-00735
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DOI: https://doi.org/10.1203/00006450-197704000-00735