ALTERATION OF CYTOPLASMIC ORGANELLES AFFECTS POLYMORPHONUCLEAR LEUKOCYTES ADHESIVENESS

Abstract

Striking spontaneous polarization of surface glycoproteins following exposure to Concanavalin A occurs in polymorphonuclear leukocytes (PWN) In the Chediak-Higashi syndrome (C-H) which is associated with the absence of microtubules as visualized by the transmission electron microscope. We now show that impaired microtubule assembly also correlated not only with reduced chemotaxis and degranulation but with decreased granulocyte adherence (GA) as well. GA was measured by filtering purified PMN over nylon fibers contained in a ppsteur pipette and quantitating the percentage of PMN adhering to the fibers. GA in normal mink and mice was 74±1% and 64%; whereas, it was reduced in C-H mink and mice to 40±1%; and 32% respectively. Drugs previously shown to inhibit microtubule assembly in PMN were examined. GA was decreased to 27±6%, 29±2% and 19±9% with 10−5M colchicine, 5 uM dlamide, 500 uM tertiary butyrylhydroperoxide respectively in human PMN compared to nonnal values of 43±2%. When 5 ug/ml of cytochnlasin B was incubated with human PMN, a drug known to inhibit microfilamcnt polymerization, GA was also reduced to 33%. These studies support the notion that impairment of either cytoplasmic microtubule or microfilament assembly will lead to alterations in the surface properties of PMN membrane which, in turn, will affect the ability of the PMN to adhere to activated surfaces.

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Allen, J., Boxer, L. & Baehner, R. ALTERATION OF CYTOPLASMIC ORGANELLES AFFECTS POLYMORPHONUCLEAR LEUKOCYTES ADHESIVENESS. Pediatr Res 11, 483 (1977). https://doi.org/10.1203/00006450-197704000-00682

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