Abstract
Neonatal platelet dysfunction is common and correlates Fith certain neonatal and maternal factors:
In addition, a thermo-labile inhibitor of platelet aggregation vac demonstrated in 9/28 unbilical vein endothelicl cells (UVECs) extracted by collogenase-IV treatment followed either by freeze-thawing or sonicution. The addition of spermine or spormidine enhanced this inhibition. Perfusion experiments were conducted in which platelet-rich plasma was pumped at vacicus rates in a pulsatile manner either through plastic tubing or with a portion of the tubing replaced by 10-15 cm. nections of fresh umbilical veins. An 8.7-fold overall reduction in platelet aggregation was observed with the insertion of this venous segment. This was most pronounced with AUT-induced aggregation but was also seen with ristocetin and epinephrine. Scannning and transmission electron micrographs demonstruted less infraction between platelets and endothelial cells followins perfusion with UVEC extracts.
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Miale, T., Nelson, R., Ryan, S. et al. PRELIMINARY INVESTIGATIONS OF ENDOTHELIAL CELL PARTICIPATION IN NEONATAL PLATELET DYSFUNCTION. Pediatr Res 11, 476 (1977). https://doi.org/10.1203/00006450-197704000-00640
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DOI: https://doi.org/10.1203/00006450-197704000-00640