Abstract
Plasma (P) cholesterol (C) levels in 181 subjects measured using a capillary blood gas-liquid chromatography(GLC) micromethod (20 μl of plasma), were compared to C levels in venous blood using ferric-chloride automated colorimetric methods (AA-1). For the GLC, 20μl of plasma was saponified with tetramethylammonium hydroxide-isopropanol (with 5 α cholestane as internal standard) and was extracted with tetrachlorethylene:methyl butyrate 1:3 v/v. After addition of water, the tetrachlorethylene lower phase was injected into the GLC and C concentration determined “by peak-height ratio comparison with reference P. In 181 subjects C (mean ±SD) for the AA-1 method (which required 5 ml plasma and venesection) was 235± 54 essentially identical to C measured by the GLC method, 235±57 mg/100ml. The correlation of the C levels by AA-1 and GLC was very close, r=0-96, p<. 001. Two-way analysis of variance revealed no differences between C by AA-1 or GLC. There were no differences between C by AA-1 or GLC for subjects with plasma triglycerides >200 mg/100 ml and those with TG<200. The close concordance of the GLC capillary method with the venous blood AA-1 method allows reduction in sample size to 20 μl plasma, negates the necessity for venesection, and facilitates lipid studies in infants and children.
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Ishikawa, T., Morrison, J., Fallat, R. et al. COMPARISON OF CAPILLARY AND VENOUS BLOOD SAMPLING FOR QUANTITATION OF PLASMA CHOLESTEROL. Pediatr Res 8, 434 (1974). https://doi.org/10.1203/00006450-197404000-00562
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DOI: https://doi.org/10.1203/00006450-197404000-00562