Abstract
A lymphocyte stimulation assay employing HSV antigens was developed because of information suggesting that impaired cellular immunity plays a role in disseminated herpetic infection of the newborn and severe herpetic infection of immunocompromised individuals. Leukocytes were obtained by Dextran sedimentation, suspended in RPMI 1640 with autologous plasma, and incubated with HSV-1 or HSV-2 antigens and control antigens. After 6 days incubation, incorporation of H3-thymidine over a 4 hr. period was measured.
When studied 1-3 days after the onset of clinical manifestations, four of five children with laboratory diagnosed herpetic gingivostomatitis showed no response to HSV-1 antigen and one showed three-fold stimulation. One to three weeks later lymphocytes from children with no initial response demonstrated 3-60 fold stimulation.
HSV-2 infected newboms showed significant stimulation with HSV-2 antigen and lesser responses to HSV-1 antigen. Cord lymphocytes from uninfected newborns showed no response to either antigen.
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Starr, S., Karatela, S., Shore, S. et al. LYMPHOCYTE STIMULATION BY HERPES SIMPLEX VIRUS (HSV) ANTIGENS IN HSV-1 OR HSV-2 INFECTED NEWBORNS AND CHILDREN. Pediatr Res 8, 419 (1974). https://doi.org/10.1203/00006450-197404000-00477
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DOI: https://doi.org/10.1203/00006450-197404000-00477