Abstract
We report the immunological reconstitution of an infant with combined immunodeficiency disease (CID) following transplantation of cells from a donor incompatible by mixed lymphocyte culture (MLC) typing.
Previous long term reconstitution in CID has been achieved by transplantation of bone marrow cells either genetically compatible by HL-A typing or more recently, in MLC.
Reconstitution has occurred in this infant with CID. No donor cells completely compatible in MLC were available. However, paternal immunocytes reactive in MLC could be eliminated in vitro with a pulse of tritiated thymidine of high specific activity. The remaining immunocytes were MLC compatible with the infant's cells. Cells were prepared in vitro in this way on three occasions (twice from peripheral blood and once from bone marrow) and transplanted intraperitoneally over a 4 month period, utilizing increasing numbers of donor cells each time.
Now at 12 months of age, the infant exhibits a moderate chronic graft versus host reaction. Immunoglobulins are normal. Cell mediated responsiveness measured in vitro by phytohemagglutinin, pokeweed, and MLC reactivity is present. Skin tests have reverted to negative. B and T lymphocytes are present. This suggests that there is a potential source of cells from non-compatible donors for reconstitution of CID.
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Sieber, O., Fulginiti, V., Durie, B. et al. IMMUNOLOGICAL RECONSTITUTION IN SEVERE COMBINED IMMUNODEFICIENCY DISEASE WITH TRANSPLANTATION FROM A NON-COMPATIBLE DONOR. Pediatr Res 8, 419 (1974). https://doi.org/10.1203/00006450-197404000-00472
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DOI: https://doi.org/10.1203/00006450-197404000-00472
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