Abstract
Quantitative evalution of gluconeogenesis in humans is difficult since liver and kidney are not suitable for repeated investigation. Therefore, gluconeogenesis in easily available tissue such as platelets was studied during incubation in buffered solution. Upon additon of 20 mM pyruvate glucose formation in the incubation medium amounted to 6.99 μmoles hr 1011 platelets and glycogen utilization was inhibited to 28.8%. Incorporation of label from pyruvate incorporated/hr 1011 platelets. These results are interpreted as evidence for the existence of gluconeogenesis in human platelets. In two patients with glycogen strorage discase type 1 incorporation of label from pyruvate-2-11C into carbohydrates was decreased to 0.95 μmoles/hr/1011 platelets. Only 7% of the total radioactivity was found to be in glucose in these patients as compared to 81% in normal, indicating that most radioactivity was incorporation of label from pyruvate-2-14C into carbohydrates by platelets of two patients with glycogen storage discase type III showed no increase in comparison to normal: however, in these patients 95% of the label was found in glucose. This finding indicates that in type III glycogenosis a greater relative part of pyruvate is incorporated into glucose than in normal. The conclusion is drawn from these studies that human plateleta might prove to be a valuable material for studying gluconeogenesis under various condition.
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Zuppinger, K., Nakamura, N., Colombo, J. et al. 67. Gluconeogenesis in human platelets. Pediatr Res 5, 97 (1971). https://doi.org/10.1203/00006450-197102000-00072
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DOI: https://doi.org/10.1203/00006450-197102000-00072