Abstract
In 1966 we showed high extent of blastogenesis in the 3-day PHA culture of peripheral blood lymphocytes from ALL (Lancet, i: 1265, 1966). The results of the PHA-responsiveness of lymphocytes obtained in ALL from different sources besides peripheral blood, such as bone marrow, lymphonotes, leukemic pleural effusion and leukemic spinal fluid, are now discussed. Concerning the peripheral blood lymphocytes, all but 2 of a total of 19 untreated patients examined at the time of diagnosis showed extent of blastogenesis from 50 to 90%. The lymphocytes from quite enlarged lymphonodes of 2 children showed normal high percentages of blasts. On the other hand, the bone marrow PHA cultures of 11 of the above mentioned 19 patients gave high extent of blastogenesis (ranging from 58 to 88%) only in 4 of them. In fact, the bone marrow of 3 patients showed blast percentages of only 1.1 11, and 11%, whereas the remaining 1 children had no lymphocyte transformation at all. Again, the lymphocytes obtained from the spinal fluid in 3 evaluable patient of 7 examined, all affected by meningeal leukemia, and from the pleural effusion in 1 patient did not undergo any blastic transformation. It appears that a quite different behaviour concering PHA blastogenesis of lymphicytes in ALL can be observed according to the source the lymphocytes come from.
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Massimo, L., Dagna-Bricarelli, F., Mori, P. et al. 65. Blastogenesis of lymphocytes from different sources in acute lymphoblastic leukemia (ALL) of children. Pediatr Res 5, 96–97 (1971). https://doi.org/10.1203/00006450-197102000-00070
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DOI: https://doi.org/10.1203/00006450-197102000-00070