Abstract
3,8,13,17-Tetramethyl-7,12-divinyl-2,18-bis-(18-hydroxyoctadecyloxycarbonyl-ethyl)porphinato zinc (BHPZn) was incorporated into the hydrophobic bilayer membrane region of liposome of dipalmitoyl phosphatidylcholine (DPPC). Thionine was added to the outer aqueous phase of the liposome suspension. Fluorescence spectra were measured to check the influence of phase of lipid bilayer on the energy transfer from BHPZn to thionine. At temperatures below the phase transition temperature (Tc) of DPPC liposome, BHPZn was assembled in the bilayer membrane and the excitation energy of BHPZn was dissipated by the static self-concentration quenching. The energy transfer efficiency to thionine was considerably low. Against this, at temperatures above the Tc, concentrated BHPZn was dispersed uniformly and reduced the self-quenching of BHPZn in the excited state. The energy transfer efficiency from porphyrin to thionine increased with heating depending on the fluidity of the lipid bilayer. The fluorescence characteristics of BHPZn reflected the phase behavior of the lipid bilayer, and was simply controlled by changing the physicochemical feature of lipids such as fluidity.
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M. P. Pileni, Chem. Phys. Lett., 71, 317 (1980).
Y. Aso, K. Kano, and T. Matsuo, Biochim. Biophys. Acta, 599, 403 (1980).
E. Tsuchida, M. Kaneko, H. Nishide, and M. Hoshino, J. Phys. Chem., 90, 2283 (1986).
T. Nomura, J. R. Escabi-Perez, J. Sunamoto, and J. H. Fendler, J. Am. Chem. Soc., 102, 1484 (1980).
J. K. Hurley, F. Castelli, and G. Tollin, Photochem. Photobiol., 32, 79 (1980).
L. Wang, E. Shouji, H. Ohno, and E. Tsuchida, Polym. Bull. (1989), in press.
B. K. Fung and L. Stryer, Biochemistry, 17, 5241 (1978).
K. Kano, T. Yamaguchi, and T. Matsuo, J. Phys. Chem., 84, 72 (1980).
M. T. Allen, L. Miola, and D. G. Whitten, J. Am. Chem. Soc., 110, 3198 (1988).
H. Takemoto, S. Inoue, T. Yasanaga, M. Sukigara, and Y. Toyoshima, J. Phys. Chem., 85, 1032 (1981).
L. Wang, T. Takahashi, H. Ohno, and E. Tsuchida, J. Macromol. Sci., Chem. Ed., A26, 481 (1989).
L. Wang, H. Ohno, and E. Tsuchida, Bull. Sci. Eng. Res. Lab., Waseda Univ., 121, 8 (1988).
M. Rotenberg and R. Margalit, Biochim. Biophys. Acta, 905, 172 (1987).
K. A. Zachariasse and D. G. Whitten, Chem. Phys. Lett., 22, 527 (1973).
T. Förster, Discus. Faraday Soc., 27, 7 (1959).
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Wang, L., Ohno, H. & Tsuchida, E. Reversible Association of Porphyrin Derivative Incorporated into Hydrophobic Region of Phospholipid Bilayer. Polym J 21, 505–510 (1989). https://doi.org/10.1295/polymj.21.505
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DOI: https://doi.org/10.1295/polymj.21.505