Abstract
Photoresposive polypeptides, poly(L-glutamic acid)s containing azobenzene-4-sulfonic acid residues in the side chains (azo·S-X-PGA, X is the mol% of the azobenzene sulfonate residue), were adsorbed onto a porous support membrane and photoinduced permeation changes of the membrane obtained were studied. The maximum amount of adsorption, nmax (g g−1), of azo·S-PGA in the membrane was shown to decrease by increasing the azobenzene sulfonate groups, X, resulting from decrease in the content of the L-glutamic acid residues which interact with the adsorption sites on the pore wall through hydrogen bonds. It was estimated that the value of nmax of the azo·S-14.1-PGA adsorbed membrane used for the permeation measurements was 1.40×10−2 g of the polymer per g of the support membrane. The filtration coefficient of the azo·S-14.1-PGA adsorbed membrane largely decreased at the pH where α-helix to coil transition of the polymer occurred, indicating that permeation of the membrane was mainly determined by the conformation of the adsorbed azo·S-PGA on the pore wall. Moreover, it was observed that photoirradiation of the membrane at adequate pHS could induce rapid decrease in Lp of the membrane within a few minute. The photoresposive behavior of the membrane can be explained in terms of the photoinduced helix to coil transition of the azo·S-14.1-PGA in the membrane. The permeability of the membrane containing azo·S-46.3-PGA, which was in the random coil state at any given pH value in the aqueous solution, was independent on pH and photoirradiation.
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Sato, M., Kinoshita, T., Takizawa, A. et al. Permeability Changes of a Porous Membrane by an Adsorbed Photoresponsive Polypeptide. Polym J 20, 761–769 (1988). https://doi.org/10.1295/polymj.20.761
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DOI: https://doi.org/10.1295/polymj.20.761