Abstract
Three component random copolypeptides consisting of N-hydroxyalkyl L-glutamine, L-glutamic acid, and L-lysine were prepared by carrying out aminolysis reactions with aminoalcohols, such as 3-amino-1-propanol (P) or 5-amino-1-pentanol (Pe), followed by crosslinking reaction with octamethylenediamine (OMDA) on starting polymer membranes consisting of γ-methyl-L-glutamate (M), L-glutamic acid (B), and L-lysine (K). The effective crosslink density was shown to be proportional to the content of the crosslinker (OMDA) in the reaction mixture. The tensile properties of these hydrophilic membranes were highly dependent on the degree of swelling in the pseudo-extracellular fluid (PECF), hydrophobicity of the side chains, and the effective charge density of membranes, and their behavior was typical of an elastomer. A higher rate of water permeability was obtained with charged membranes than non-charged and/or compensated charged membranes with the same order of the degree of swelling in PECF. Biodegradation of the samples in vitro by pronase E and papain indicated that the degradation could be regarded as a bulk rather than a surface phenomenon. The rate of degradation was also highly dependent on the degree of swelling of membranes, as well as on the hydrophobicity and effective charge density of side chains of the samples.
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Hayashi, T., Nakanishi, E. & Nakajima, A. Preparation and Properties of Charged Copolypeptide Membranes as Biodegradable Materials. Polym J 19, 1025–1032 (1987). https://doi.org/10.1295/polymj.19.1025
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DOI: https://doi.org/10.1295/polymj.19.1025