Abstract
The emission anisotropy of the fluorescence depolarization of rhodamine B chemically bound to the terminal amino group of poly(γ-benzyl L-glutamate) (PBLG) was measured in various helicogenic solvents (benzene, dioxane, etc.). The apparent mean rotational relaxation time of the chromophore attached to chain end corresponded to the aggregation behavior of PBLG in various helicogenic solvents. This shows that the terminal rhodamine B group as a chromophore is tightly bound to the PBLG chain end. The aggregation and helix–coil transition behavior of PBLG has been studied in various solvent mixtures (benzene–dichloroacetic acid, benzene–N,N-dimethylformamide, etc.). In the series of highly aggregation-solvents such as benzene and dioxane, PBLG is aggregated up to an N,N-dimethylformamide content of about 80—90%. The aggregation-breaking (dissociation) and helix-breaking ratios as parameters of aggregation and helix–coil transition behavior of PBLG are briefly discussed for various solvent mixtures.
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Ushiki, H., Mita, I. Study on the Fluorescence Depolarization of a Chromophore Attached to Polymer Chain End II. Aggregation and Helix–Coil Transition Behavior of Poly(γ-benzyl L-glutamate) in Various Mixed Solvents. Polym J 16, 751–759 (1984). https://doi.org/10.1295/polymj.16.751
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DOI: https://doi.org/10.1295/polymj.16.751