Abstract
X-Ray diffraction measurements were made on fibers of poly[γ-(o-chlorobenzyl) L-glutamate], poly[γ-(m-chlorobenzyl) L-glutamate], and poly[γ-(p-chlorobenzyl L-glutamate]. All reflections of the poly[γ-(o-chlorobenzyl) L-glutamate] fibers were indexed on an orthorhombic lattice of a=26.50 Å. b=30.60 Å, and c=27.02 Å. The observed density was 1.367 g cm−3 as compared with the calculated value of 1.382 g cm−3. All reflections of the poly[γ-(m-chlorobenzyl) L-glutamate] fibers were indexed on a hexagonal lattice of a=b=15.50 Å and c=27.10 Å. The observed density of 1.356 g cm−3 was larger than the calculated value of 1.343 g cm−3. All reflections of the poly[γ-(p-chlorobenzyl) L-glutamate] fibers were indexed on an orthorhombic lattice of a=15.24 Å, b=26.40 Å, and c=10.67 Å. The observed density of 1.362 g cm−3 was comparable with the calculated value of 1.371 g cm−3. By applying the methods of the cylindrical Patterson map and equatorial Fourier map, the position of the chlorine atom at the end of the side chain was estimated. The results obtained indicated that the radial distance of the chlorine atom from the helix axis is about 4 Å, 6 Å, and 10 Å for poly[γ-(o-chlorobenzyl) L-glutamate], poly[γ-(m-chlorobenzyl) L-glutamate], and poly[γ-(p-chlorobenzyl) L-glutamate], respectively. These results are interpreted in terms of side chain conformation.
Similar content being viewed by others
Article PDF
References
E. M. Bradbury, A. R. Downie, A. Elliott, and W. E. Hanby, Proc. R. Soc. London, Ser. A, 259, 110 (1960).
Y. Takeda, Y. Iitaka, and M. Tsuboi, J. Mol. Biol., 51, 101 (1970).
M. Osanai and K. Hikichi, Polym. J., 13, 817 (1981).
M. Osanai and K. Hikichi, Biopolymers, 19, 1099 (1980).
L. Brown and I. F. Trotter, Trans. Faraday Soc., 52, 537 (1956).
G. D. Fasman, Ed., “Poly-α-Amino Acids,” Marcel Dekker, Inc., New York, N. Y., 1967, p 1.
F. Colonna-Cesari and S. Permilat, Polymer, 17, 267 (1976).
C. H. Bamford, L. Brown, A. Elliott, W. E. Hanby, and I. F. Trotter, Proc. R. Soc. London, Ser. B, 141, 49 (1953).
A. Elliott, R. D. B. Fraser, and T. P. MacRae, J. Mol. Biol., 11, 821 (1965).
C. H. MacGillavry and E. M. Bruins, Acta Cryst., 11, 199 (1967).
Y. Mitsui, Y. Iitaka, and M. Tsuboi, J. Mol. Biol., 24, 15 (1967).
L. Pauling and R. B. Corey, Proc. Natl. Acad. Sci. U.S.A., 37, 235 (1951).
C. H. Bamford, W. E. Hanby, and F. Happey, “Synthetic Polypeptides,” Academic Press, New York, N.Y., 1956, p 267.
R. D. B. Fraser, B. S. Harrap, R. Ledger, T. P. MacRae, F. H. C. Stewart, and E. Suzuki, Biopolymers, 5, 797 (1967).
N. Sasaki, A. Tsutsumi, K. Hikichi, Y. Konishi, and M. Hatano, Polym. J., 11, 583 (1979).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Osanai, M., Hikichi, K. X-Ray Study of the Substitution Effect in Poly[γ-(o-chlorobenzyl) L-glutamate], Poly[γ-(m-chlorobenzyl) L-glutamate], and Poly[γ-(p-chlorobenzyl) L-glutamate] in the Solid State. Polym J 14, 411–422 (1982). https://doi.org/10.1295/polymj.14.411
Issue Date:
DOI: https://doi.org/10.1295/polymj.14.411