Abstract
The CD difference spectrum was applied to the elucidation of the conformational change in RNA as well as the estimation of its base pair content. First, it was revealed that the RDV RNA recooled form was composed of the fully reconstructed G–C pairs and partially destroyed A–U pairs, being in good agreement with the result of RNase treatment. Second, the CD difference spectrum of unfractionated tRNA from an extreme thermophile was quite similar to that of tRNAfmet from E. coli and random copolymer poly (G, C), showing that the thermophile tRNA is rich in G–C pairs and is consistent with the result of Tm measurement. The limitedly hydrolyzed fragments of tRNAfmet by RNase T1 and their equimolar complexes were also studied. The CD and CD difference spectra of 3′-three-quarter molecules (fragment L, M3, M2) were almost identical but those of their equimolar complexes with 5′-quarter molecule (fragment N) were different from each other. Especially N+M2 revealed a quite different CD pattern from the rest. By combining the work of electrophoresis it was deduced that some false complexes were formed in N+M2 due to incompleteness of base pairs in the diHU arm.
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Watanabe, K., Seno, T., Nishimura, S. et al. The Study of Circular Dichroic Difference Spectra Applied to Polynucleotides and Ribonucleic Acids. Polym J 4, 539–552 (1973). https://doi.org/10.1295/polymj.4.539
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DOI: https://doi.org/10.1295/polymj.4.539