Abstract
Helix-initiation parameter σ and enthalpy change ΔH for helix–coil transition of poly(γ-benzyl-L-glutamate) in mixtures of dichloroacetic acid and ethylene dichloride were determined at fixed solvent conditions from the dependence of helical content of the dissolved polypeptide on its molecular weight. The values of \\sqrt{σ} obtained were substantially independent of solvent composition and temperature over the range where the substantial portion of the transition occurred, yielding (0.95±0.13)×10−2 on the average. This value stood in close agreement with that evaluated from calorimetric measurements by Ackermann and Neumann. On the other hand, ΔH depended slightly on solvent composition and had values between those of Ackermann and Neumann and of Karasz and O’Reilly.
Similar content being viewed by others
Article PDF
References
B. H. Zimm, P. Doty, and K. Iso, Proc. Nat. Acad. Sci., U.S., 45, 1601 (1959).
B. H. Zimm and J. K. Bragg, J. Chem. Phys., 31, 526 (1959).
K. Okita, A. Teramoto, and H. Fujita, Biopolymers, 9, 717 (1970).
F. E. Karasz and J. M. O'Reilly, Biopolymers, 4, 1015 (1966).
T. Ackermann and E. Neumann, Biopolymers, 5, 649 (1967).
G. Giacometti, A. Turolla, and R. Boni, Biopolymers, 6, 441 (1968).
A. Kagemoto and R. Fujishiro, Biopolymers, 6, 1753 (1968).
E. R. Blout and R. H. Karlson, J. Am. Chem. Soc., 78, 941 (1956).
K. Nakagawa, M. S. Thesis, Osaka University, 1968.
R. D. Lundberg and P. Doty, J. Am. Chem. Soc., 79, 3961 (1957).
T. Yamashita, M. S. Thesis, Osaka University, 1966.
W. Moffitt and J. T. Yang, Proc. Nat. Acad. Sci., U.S., 42, 596 (1956).
G.D. Fasman, in "Methods in Enzymology," Vol. VI, S. P. Colowick and N. O. Kaplan Ed., Academic Press, New York, 1963, p 928.
A. Nakajima and T. Hayashi, Bull. Inst. Chem. Res., Kyoto Univ., 46, 62 (1968).
A. Teramoto, K. Nakagawa, and H. Fujita, J. Chem. Phys., 46, 4197 (1967).
A. Teramoto, T. Yamashita, and H. Fujita, J. Chem. Phys., 46, 1919 (1967).
P. Doty and J. T. Yang, J. Am. Chem. Soc., 78, 498 (1956).
P. Urnes and P. Doty, Adv. Protein Chem., 16, 401 (1961).
J. Y. Cassim and E. W. Taylor, Biophys. J., 5, 5533 (1965).
A. Teramoto, T. Norisuye, and H. Fujita, Polym. J., 1, 55 (1970).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Norisuye, T., Matsuoka, M., Teramoto, A. et al. Solution Properties of Synthetic Polypeptides. X. Transition Parameters for Poly(γ-benzyl-L-glutamate). Polym J 1, 691–699 (1970). https://doi.org/10.1295/polymj.1.691
Issue Date:
DOI: https://doi.org/10.1295/polymj.1.691