CRIPTO (CR-1, TDGF1) is a cell surface/secreted oncoprotein actively involved in development and cancer. Here, we report that high expression of CRIPTO correlates with poor survival in stratified risk groups of prostate cancer (PCa) patients. CRIPTO and its signaling partner glucose-regulated protein 78 (GRP78) are highly expressed in PCa metastases and display higher levels in the metastatic ALDHhigh sub-population of PC-3M-Pro4Luc2 PCa cells compared with non-metastatic ALDHlow. Coculture of the osteotropic PC-3M-Pro4Luc2 PCa cells with differentiated primary human osteoblasts induced CRIPTO and GRP78 expression in cancer cells and increases the size of the ALDHhigh sub-population. Additionally, CRIPTO or GRP78 knockdown decreases proliferation, migration, clonogenicity and the size of the metastasis-initiating ALDHhigh sub-population. CRIPTO knockdown reduces the invasion of PC-3M-Pro4Luc2 cells in zebrafish and inhibits bone metastasis in a preclinical mouse model. These results highlight a functional role for CRIPTO and GRP78 in PCa metastasis and suggest that targeting CRIPTO/GRP78 signaling may have significant therapeutic potential.
Prostate cancer (PCa) is the second most common cancer in men worldwide.1 While current treatments of primary tumors are initially very effective, these beneficial responses are often followed by tumor recurrence and incurable bone metastases. Therefore, identifying molecular mediators of PCa relapse and metastasis will aid in the development of therapies for this deadly phase of the disease.
CRIPTO (TDGF1, CRIPTO-1) is a small, GPI-anchored/secreted fetal oncoprotein that has important roles in regulating stem cell differentiation, embryogenesis, tissue growth and remodeling.2 CRIPTO promotes transformation, migration, invasion and angiogenesis and its misregulation can contribute to cancer development and progression in multiple malignancies, including breast cancer and PCa, which are both characterized by osteotropism in their metastatic stage.3, 4 CRIPTO modulates crucial pathways that regulate bone metastasis such as the tumor growth factor-β (TGF-β) pathway5 and functions as an obligatory coreceptor for Nodal, a TGF-β superfamily member that promotes epithelial-to-mesenchymal transition (EMT) in PCa.5, 6, 7 Glucose-regulated protein 78 (GRP78) was identified as a CRIPTO-binding protein and essential mediator of CRIPTO signaling.8, 9, 10 GRP78 is well established as a key survival factor in development and cancer 8, 9 and, notably, upregulation of GRP78 has been associated with the development of castration-resistant PCa.11 While CRIPTO was reported to impact primary human prostate adenocarcinomas,6 its role in driving castration-resistant PCa and PCa bone metastasis remains unknown.
Here, we investigated the roles of CRIPTO and GRP78 in aggressive, metastatic human PCa cells both in vitro and in vivo using an embryonic zebrafish model and a preclinical mouse model of experimentally induced PCa bone metastasis. We found that CRIPTO and GRP78 are upregulated in clinical samples of PCa metastases from human patients and in the highly metastatic ALDHhigh stem/progenitor-like sub-population of a human castration-resistant PCa cell line.12, 13 We further demonstrate that knockdown of CRIPTO or GRP78 in these cells decreases the size of the stem/progenitor-like sub-population and also inhibits their extravasation following inoculation into zebrafish and their metastatic potential in a preclinical mouse model of bone metastasis in vivo. Taken together, these findings provide new evidence that CRIPTO and GRP78 may drive metastatic PCa and highlight the therapeutic potential of targeting the cell surface CRIPTO/GRP78 complex for the treatment of this deadly disease.
CRIPTO is highly expressed in metastases from human PCa patients and correlates with poor survival
CRIPTO and its signaling partner GRP78 have been shown to have important roles in tumor development and bone metastasis.14 We investigated the correlation of survival with CRIPTO expression in independent sets of public PCa data sets (GSE21032 and GSE1064515, 16). In both data sets, CRIPTO expression was associated with poor prognosis (Figures 1a and b (top); hazard ratio (HR)=1.87 and P=0.001 for GSE10645 and HR=2.2 and P=0.06 for GSE21032). Additionally, in both data sets, the expression of CRIPTO in stratified risk groups was significantly higher in the high- vs low-risk group (Figures 1a and b (bottom); P=1.13e−103 for GSE10645 and P=3.68e−23 for GSE21032). We further investigated the expression of CRIPTO and GRP78 in the Ramaswamy Multi-cancer data set from Oncomine (Compendia Bioscience, Waltham, MA, USA), which compares PCa metastasis to primary sites in 76 samples17 and includes quantified differential gene expression patterns between human primary tumors and metastatic lesions arising from corresponding primary site. Additionally, to investigate the levels of CRIPTO in site-specific metastases, we also analyzed a previously published data set (Chandran prostate GSE6752) where 31 prostate specimens (10 primary and 21 hormone-refractory metastatic) were included.18 We found that CRIPTO and GRP78 are significantly upregulated in metastatic lesions arising from human prostate tumors, compared with primary sites in human PCa (Figures 1c, P=7.15e−4 for CRIPTO and P=0.03 for GRP78; Supplementary Figures 1A and B). Subsequently, we investigated the expression of CRIPTO in PCa bone metastases by immunostaining of paraffin-embedded sections of bone metastases freshly isolated from patients. We detected significant expression of CRIPTO and colocalization of CRIPTO with cytokeratin-18 in serial sections from each of the 13 specimens analyzed (Figure 1d). Taken together, these data demonstrate that CRIPTO is selectively expressed in aggressive and metastatic PCa and that its expression correlates with poor prognosis.
Coculture with primary human osteoblasts augments the ALDHhigh sub-population, upregulates CRIPTO and GRP78 and promotes the metastatic phenotype of PCa cells
We previously reported that the ALDHhigh sub-population of PC-3M-Pro4Luc2 cells displays stem/progenitor-like properties and is highly metastatic relative to its ALDHlow counterpart.12 CRIPTO and GRP78 are expressed in human PCa cell lines (PC-3M-Pro4Luc2 and C4-2B cells) (Figures 2a–c) and given their known roles in regulating tumor aggressiveness, we tested if CRIPTO and GRP78 are selectively expressed in the ALDHhigh sub-population. Quantitative real-time PCR (qRT–PCR) (Figure 2d) and protein analysis (Figures 2e and f) on selected sub-population of cells isolated after viable cell sorting showed that GRP78 levels were indistinguishable in both sub-populations but that CRIPTO was more highly expressed in the ALDHhigh sub-population than in the ALDHlow (P<0.01 for CRIPTO). This result is consistent with a role for CRIPTO in promoting PCa growth and metastasis.
In PCa and other cancers, the osteoblastic microenvironment functions as premetastatic niche by attracting bone-metastasizing tumor cells.19, 20, 21 We developed a model of the bone metastatic niche in which primary human osteoblasts are cocultured with PC-3M-Pro4Luc2 cells in vitro. Differentiation of the human osteoblasts was confirmed by Alizarin red staining (Supplementary Figure 2A). We found that in the presence of osteoblasts, the size of the ALDHhigh sub-population increases markedly compared with the size of the ALDHhigh sub-population in PCa cells alone (coculture=60 vs control=15%, P<0.05) (Figure 2g). This effect appeared to be osteoblast-specific as coculture of PCa cells with a non-osteoblast cell line (HEK293T cells) failed to reproduce the significant increase in the percentage of ALDHhigh cells (Supplementary Figure 2b). Moreover, mRNA analysis after viable cell sorting of fluorescently labeled PC-3M-Pro4Luc2 cells (cocultured cells compared with control) showed significant increase in CRIPTO (P<0.05) and GRP78 expression (Figure 2h). In addition, conditioned medium (CM) generated in the absence of serum, collected from osteoblasts, increased the migratory capability of PC-3M-Pro4Luc2 cells and the increase was directly proportional to the concentration of the CM used (P<0.001 for 50 CM+50% not-CM vs control; P<0.001 for 100% CM vs control and P<0.001 for 100 CM vs 50% CM; Figure 2i). In line with these observations, administration of osteoblast CM led to the acquisition of a motile, mesenchymal phenotype in PC-3M-Pro4Luc2 PCa cells as indicated by a decrease in the mRNA expression of the epithelial marker E-cadherin (P<0.05), a concomitant increase in the mesenchymal markers ZEB1 and ZEB2 (P<0.05 for both genes) (Supplementary Figure 2C) and a significant reduction of the ratio E-cadherin/vimentin (P<0.05) and ratio E-cadherin/N-cadherin (Figure 2j). Taken together, these data indicate that secreted factors derived from osteoblasts promote the metastatic phenotype of PCa cells and increases their expression of CRIPTO and GRP78.
CRIPTO and GRP78 maintain aggressiveness of human PCa cells in vitro
To test the function of CRIPTO and GRP78 in human PCa cells, we generated stable knockdown lines in PC-3M-Pro4Luc2 and C4-2B cells and validated reduced expression of CRIPTO and GRP78 by qRT–PCR, western blot and FACS analysis (Figures 3a and b and Supplementary Figures 3A–E). As previously shown by others in PC3 cells and consistent with the extensive post-translational modification of CRIPTO, we detected two protein bands of ~17 and 25 kDa5, 22 (Figure 3b). CRIPTO knockdown lines (short hairpin RNA (shRNA) no. 2 and 3) both show decreased cell proliferation (P<0.001 at 24 h for shRNA no. 2 and P<0.001 at 24, 48 and 72 h for shRNA no. 2 and 3) (Figure 3c). We confirmed the acquisition of this phenotype upon CRIPTO silencing by an additional pool of independent siRNAs targeting CRIPTO (Supplementary Figures 3F and 3G). No effect on cell proliferation was observed in the C4-2B CRIPTO knockdown cells (Supplementary Figure 4A). The PC-3M-Pro4Luc2 CRIPTO knockdown cells also had a significantly reduced percentage of metastatic, stem cell-like ALDHhigh cells relative to non-targeted (NT) control cells (P<0.05 for shRNA no. 2 and P=NS for shRNA no. 3; Figure 3d). GRP78 knockdown similarly displayed reduction of the size of ALDHhigh sub-population of cells (P<0.05 for shRNA no. 1 and P=NS for shRNA no. 2) (Supplementary Figure 4B).
We previously reported that the ALDHhigh sub-population of PC-3M-Pro4Luc2 is enriched for cells with increased clonogenicity and migratory properties relative to the ALDHlow cells.12 Consistent with our ALDEFLUOR data, we show that PC-3M-Pro4Luc2 CRIPTO knockdown with shRNA no. 2 significantly reduces clonogenicity, as measured by the number and area of colonies produced (P<0.05) (Supplementary Figure 4C). This effect appeared to be specific since transfection of a non-targetable mouse CRIPTO expression construct resulted in significant rescue of the loss of clonogenicity caused by the CRIPTO shRNA (P<0.001 for colony number and P<0.05 for colony area) (Figure 3e). GRP78 knockdown in PC-3M-Pro4Luc2 human PCa cells also resulted in a decrease in the number of colonies (P<0.05) and a similar trend was shown for colony area (Supplementary Figure 4D). Finally, CRIPTO knockdown significantly reduced the migratory capability of both cell lines (P<0.05 and P<0.01 respectively; Figures 4a and b), these findings were confirmed by an independent pool of siRNAs (Supplementary Figure 4E). Significant rescue of the effects of shRNA knockdown could be again achieved by the non-targetable CRIPTO expression construct solely in PC-3M-Pro4Luc2 cells (Figures 4c and d). GRP78 knockdown in PC-3M-Pro4Luc2 cells also resulted in a significant reduction of migratory potential (P<0.001 for both shRNAs) (Supplementary Figure 4F). Additionally, CRIPTO knockdown in PC-3M-Pro4Luc2 cells significantly reduced the migration towards osteoblast CM (Supplementary Figures 5A and B). Taken together, these data suggest that CRIPTO and GRP78 are required to maintain an aggressive phenotype of PC-3M-Pro4Luc2 cells.
CRIPTO promotes EMT and invasiveness of human PCa cells
EMT is strongly associated with tumor cell invasion and CRIPTO was recently reported to promote EMT in human PCa.6 Consistent with this study, we found that CRIPTO overexpression in PC-3M-Pro4Luc2 and C4-2B cells (Supplementary Figures 6A and B) causes a strong and significant downregulation of the epithelial marker E-cadherin (P<0.05) at the mRNA level in both cell lines and an increase in the mesenchymal markers vimentin, SNAIL2 and TWIST in PC-3M-Pro4Luc2 cells (P<0.05, P<0.05 and P<0.01, respectively) and ZEB1 in C4-2B cells (Supplementary Figures 6c and d). CRIPTO overexpression caused a strong and significant decrease in the ratio of E-cadherin/vimentin (P<0.01) in both PCa cell lines (Figures 4e and f). Taken together, these data support a role for CRIPTO in promoting EMT and the invasive phenotype in PC-3M-Pro4Luc2 and C4-2B cells. Additionally, and consistent with previous reports,8, 23 we have found that CRIPTO modulates TGF-β signaling in our model system (Supplementary Figure 6E). These data are consistent with previous reports documenting extensive interaction of CRIPTO with the TGF-β pathway and other signaling networks.24, 25
We have previously shown that zebrafish can be used to evaluate effectively migration and invasion of human PCa cells and the interaction between PCa cells and the vasculature at the single-cell level in vivo.13, 26 Clear detection of extravasating tumor cells in this system is facilitated by the fact that the vascular system of zebrafish embryos is completely functional and the embryos are transparent.27 We tested the role of CRIPTO and GRP78 in PCa cell extravasation and metastasis by injecting fluorescently labeled PC-3M-Pro4Luc2 cells with CRIPTO or GRP78 knocked down into the circulatory system of zebrafish embryos.13 In the first hours, disseminated cells arrested in the host vasculature and then we observed extravasation from 12 hpi (hours postimplantation) and perivascular tumor cells in multiple foci including the intersegmental vessels, the optic veins, the dorsal aorta and the caudal vein. The perivascular tumor cells invaded the neighboring tail fin exclusively at the posterior ventral end of the hematopoietic tissue. At day 4 postimplantation (4 dpi), CRIPTO knockdown caused a significant reduction in extravasation and tumor growth compared with control cells with scrambled shRNA (Figures 5a–c). Similarly, GRP78 knockdown cells displayed a significant reduction in the tumor growth into the tail fin after invasion from hematopoietic tissue compared to control cells (Supplementary Figures 7A and B). However, invasion was not significantly different in GRP78 knockdown cells compared with control cells (Supplementary Figure 7C). Taken together, these data support our in vitro findings and reinforce the hypothesis that CRIPTO/GRP78 signaling has an important role in the maintenance of an invasive and aggressive phenotype in human PCa.
CRIPTO knockdown decreases metastasis formation in preclinical mouse model of PCa bone metastasis
We previously demonstrated that intracardiac injection of luciferase-expressing PC-3M-Pro4Luc2 cells in mice results in bone metastasis.12 Here, we used this preclinical mouse model to test the role of CRIPTO in mediating the metastatic activity of these PCa cells. CRIPTO knockdown cells or control cells with a scrambled shRNA were injected into the left cardiac ventricle of nude mice (Balb/c nu/nu) and bioluminescence, which reflects tumor size, was measured weekly for the course of the experiment. The same batch of cells injected was functionally tested in vitro (Supplementary Figure 8A). Quantification of bioluminescent images (Figures 6a and b, week 5, P<0.05) showed significant reduction in metastasis formation and the total number of metastases (Figure 6c, week 5, P<0.01), specifically bone metastases (Figure 6d, week 5, P<0.01), in mice inoculated with CRIPTO knockdown cells compared with mice injected with control cells. No effect on weight of the animals was measured (Supplementary Figure 8B). This result is consistent with the other findings outlined above and suggests that CRIPTO is required for bone metastasis in a mouse model of human PCa.
This study presents evidence for a role of CRIPTO and GRP78 in the regulation of the invasive program of PCa cells that maintains stem cell-like and aggressive phenotypes in human PCa. Our demonstration that CRIPTO is strongly upregulated in high-risk patient groups compared with low-risk groups and that it correlates with poor survival highlight the significance of these proteins in metastatic PCa. Importantly, the selective expression of CRIPTO and GRP78 in PCa metastases was substantiated by the analysis of publicly available data sets17, 18 and reinforced by our analysis on 13 samples of PCa bone metastasis derived from castration-resistant PCa patients.
CRIPTO/GRP78 signaling is known to regulate stem cells and tumor cells25 and our results suggest that this signaling may promote the acquisition of a metastatic phenotype in PCa. This phenotype includes the ability of PCa cells to invade the supportive stroma and neighboring tissues to allow subsequent formation of bone metastases at distant sites.28 CRIPTO/GRP78 signaling may also have a specific role in metastasis by facilitating initial colonization of the bone by PCa cells. Remarkably, we also observe a decline in the number of bone foci in the first 10–15 days of the mouse metastasis experiments, followed by much stronger growth of control tumor cells in the bone relative to the CRIPTO knockdown cells. These data are in line with our previous study, which shows a similar kinetic29 and support the hypothesis that only a small percentage of prostate cancer cells possess the ability to reach efficiently the bone, survive in the bone microenvironment and establish a new growth. Our data further suggest that this sub-population of PCa cells has stem/progenitor-like properties that depend on CRIPTO expression.30
The ALDHhigh sub-population of PC-3M-Pro4Luc2 cells is enriched for tumor-initiating cells with metastatic potential and generally accounts for a small percentage of all tumor cells.12, 13 Here, we show that these highly metastatic ALDHhigh cells have higher levels of CRIPTO protein expression compared with non-stem cell-like, non-metastatic ALDHlow cells. This finding supports our hypothesis that CRIPTO is restricted to a small sub-population of cells characterized by high metastatic ability, similar to what was recently shown in breast cancer.31 Our results also support the notion that CRIPTO signaling promotes EMT6, 7 and the migratory and invasive phenotype in PCa cells associated with a switch from a sessile, epithelial state to a motile, mesenchymal phenotype. Indeed, transcriptional analysis following CRIPTO overexpression reveals the emergence of an 'EMT signature' characterized by a marked reduction in the expression of the epithelial marker E-cadherin paralleled by a significant increase in the expression of the mesenchymal markers vimentin, Snail2 and Twist.
The activation of the bone stroma by metastatic cells alters the physiological balance between osteoblast-mediated bone formation and osteoclast-mediated bone resorption during bone metastatic colonization.32 Strikingly, we found that coculture of PCa cells with human osteoblasts, which are important cellular constituents of the bone metastatic niche,32 induced a significant increase in CRIPTO and GRP78 mRNA expression in the tumor cells. Osteoblasts have previously been reported to promote the aggressiveness of osteolytic human PCa cells in vitro.19 These findings are in line with our data showing that osteoblasts promote the metastatic phenotype of PCa cells by causing expansion of the ALDHhigh sub-population, increasing tumor cell migration and inducing expression of CRIPTO and GRP78. In light of previous studies demonstrating that CRIPTO binds cell surface GRP78 and that this interaction is required for CRIPTO signaling, our results suggest that CRIPTO and GRP78 function cooperatively to regulate the interaction between tumor cells and osteoblasts within the bone microenvironment. However, given the complexity of the bone metastatic niche, we focused primarily on the role of CRIPTO and GRP78 in the maintenance of an aggressive and metastatic phenotype in PCa cells. Additional experiments are required to elucidate the interactions between PCa cells and the different components of the bone microenvironment in mechanistic detail.
Although PCa is manageable if detected early, the castration-resistant stage of the disease is associated with high morbidity and mortality because of bone metastases. Therefore, it is critical to identify and target pathways that contribute to the dissemination and generation of PCa bone micrometastases. In this study, we demonstrate that CRIPTO and its signaling mediator GRP78 may have pivotal, functional roles in the acquisition and maintenance of an invasive, metastatic phenotype in human prostate cancer (see schematic representation in Figure 7). Therefore, CRIPTO and GRP78 represent compelling molecules for targeting and monitoring highly aggressive stem/progenitor-like PCa cells to prevent the advancement of PCa towards the castration-resistant and metastatic stage.
Materials and methods
Human osteotropic PCa PC-3M-Pro4,13, 29, 33 C4-2B cells34 and HEK293T cells35 were maintained as described previously.29 Human callus tissue from patients in good health was used to freshly isolate osteoblast precursor cells. Time to callus harvest ranged from 2 to 3 weeks. Indications for surgery were nascent (impending) malunion, failure of fixation or fractures that were operated on in a delayed manner. All fractures have subsequently successfully healed. Briefly, tissue was minced in a solution of 2 mg/ml collagenase (Millipore, Billerica, MA, USA) for 3 h at 37 °C and vortexed every 30 min for 1–2 min. The digested tissue was centrifuged for 5 min two times at 1000 r.p.m. (room temperature) in a media (Dulbecco's modified Eagle's medium, Glutamax MEM Non-Essential Amino Acids (1 × ; Gibco-Thermo Scientific, Waltham, MA, USA), P/S). Pellet was resuspended in 0.25% Trypsin solution (Invitrogen, Waltham, MA, USA) for 1 h at 37 °C. The digested tissue was centrifuged at 1000 r.p.m. for 5 min in media. The cells were plated in osteoblast precursor media (Dulbecco's modified Eagle's medium with 10% fetal calf serum, 1% penicillin–streptomycin and 1% MEM Non-Essential Amino Acids; Gibco-Thermo Scientific), passaged ~80% confluency and differentiated at confluence: ascorbic acid (50 mg/ml; Merck, Darmstadt, Germany) was added in Dulbecco's modified Eagle's medium with 10% fetal calf serum, 1% penicillin–streptomycin and 1% MEM non-essential amino acids (Gibco-Thermo Scientific). Upon detection of nodules (day 11), the medium was supplemented with β-glycerolphosphate (5 mM; Sigma, St Louis, MO, USA) and 100 nM dexamethasone. After 3 weeks, cells were fixed with 4% paraformaldehyde and analyzed for osteogenesis with Alizarin Red staining (ICN Biomedicals, Santa Ana, CA, USA).36 CM was generated with incubation time of 48 h, in the absence of serum to prevent alteration of downstream assays. Cells were maintained at 37 °C with 5% CO2.
Suppressing CRIPTO and GRP78 with shRNAs
shRNAi for CRIPTO-1 (TRCN004889, TRCN004890 and TRCN004891) and HSPA5 (TRCN231123, TRCN218611) were obtained from Sigma’s MISSION library. Lentiviral infection and selection were performed as described previously.37 Scrambled shRNA (SHC002, NT or control) with the lack of homology for any mammalian mRNA sequence was used as control.
Suppressing CRIPTO expression with siRNAs
Human CRIPTO siRNAs (SR304769A, SR304769B, SR304769C) and negative controls (SR30004, non-targeting NT) where purchased from OriGene Technologies (Rockville, MD, USA). PC-3M-Pro4-luc2 were transfected with a pool of three unique 27mer siRNA duplexes (100 nM each) or (NT) control (300 nM) siRNA. Proliferation at different time points (0, 24, 48 and 72 h) and migration were measured 48 h after transfection. Data are representative of four replicates.
Flow cytometry and aldehyde dehydrogenase assay
A total of 900 000 PC-3M-Pro4Luc2 cells fluorescently labeled with PKH26 Red Fluorescent Cell Linker (Sigma-Aldrich, St Louis, MO, USA) were seeded in a 10 cm dish on a layer of confluent and differentiated human osteoblast. After 48 h, cells were washed with phosphate-buffered saline (PBS), 1 mM EDTA and harvested using trypsin. Fluorescent PC-3M-Pro4Luc2 cells were separated from osteoblast with BD FACS ARIA (BD Biosciences, Franklin Lakes, NJ, USA). Non-labeled PC-3M-Pro4Luc2 cells, labeled PC-3M-Pro4Luc2 tumor-only and osteoblast-only cells were included as controls. Aldehyde dehydrogenase (ALDH) activity was measured using ALDEFLUOR Kit (StemCell Technologies, Durham, NC, USA).12 For knockdown validation, cells were harvested and incubated with CRIPTO antibody (FAB2772P, clone no. 89633, hCR-1-PE; R&D, Minneapolis, MN, USA) as per the manufacturer’s protocol. Data were analyzed with FCS Express Software (De Novo Software, Los Angeles, CA, USA).
Proteins were extracted with RIPA buffer and quantified using Pierce Protein Quantification Assay (ThermoFisher Scientific, Waltham, MA, USA). Ten micrograms of samples were separated by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transferred to a blotting membrane using standard techniques. Signal was detected after incubation with 1:1000 primary antibody (anti-CRIPTO, clone no. PBL6900; Spike et al.31) and with 1:10 000 secondary horseradish peroxidase antibody (Promega, Madison, WI, USA).
CRIPTO construct, generated as described previously,23 was transfected in PC-3M-Pro4Luc2 and C4-2B cells with Lipofectamine 2000 (Life Technologies, Waltham, MA, USA) or with Fugene HD (Promega), respectively, according to the supplier’s protocol. Data are representative of at least two independent experiments.
RNA isolation and real-time quantitative PCR
Total RNA was isolated with Trizol Reagent (Invitrogen) and cDNA was synthesized by reverse transcription (Promega) according to the protocol. qRT–PCR was performed with Bio-Rad CFX96 (Bio-Rad, Hercules, CA, USA). Gene expression was normalized to glyceraldehyde-3-phosphate dehydrogenase, hypoxanthine-guanine phosphoribosyltransferase and actin. Primers are listed in Supplementary Table 1.
Luciferase reporter assay
A total of 10 000 PC-3M-Pro4 cells were seeded in a 24-well plate and Lipofectamine 2000 (Invitrogen) was used according to the manufacturer’s protocol. Hundred nanograms of CAGA-Luc (TGF-β reporter38) and 10 ng of CAGGS-Renilla were transfected in combination of CRIPTO overexpression (100 ng construct per well) 24 h before assessment of Luciferase. Firefly and Renilla luciferase were measured with Dual Luciferase Assay (Promega). Data are shown as relative light units and representative of at least two independent experiments. Firefly luciferase is normalized for Renilla luciferase.
Cells were seeded at density of 1500 cells per well and allowed to grow for 24, 48 and 72 h. Proliferation was assessed with 3-(4,5 dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium after 2 h incubation at 37 °C (CellTiter96 Aqueous Assay; Promega). Data were normalized for the number of cells. N=5 per condition, performed in duplicate experiments.
Transwell migration assay
Migration assays were performed using transwells (8 mm; Corning LifeSciences, New York, NY, USA).13 For the experiments with CM from osteoblast, this was diluted in the lower chamber 1:2 with medium (50% condition) or administered undiluted (100% condition). Data are representative of at least two independent experiments, respectively.
Colony formation assay
A total of 100 cells were seeded in 2 ml of medium supplemented with 10% FCII. After 2 weeks, cells were fixed with 4% paraformaldehyde, colonies stained with 0.1% crystal violet (Sigma-Aldrich) and quantified.39 Data are representative of at least three independent experiments.
Zebrafish maintenance, embryo preparation and tumor cell inoculation
Tg(fli1:GFP)i114 zebrafish line40, 41 was handled and maintained according to local animal welfare regulations to standard protocols (http://www.ZFIN.org). Two days postfertilization, dechorionized zebrafish embryos were anesthetized and injected with PC-3M-Pro4Luc2 cells fluorescently labeled as described previously.13, 42 Quantification of the data was strictly standardized (automated pixel count of high-resolution images; criteria for quantification of extravasation are >3 cells extravasating per embryo). Zebrafish embryos (including nonimplanted controls) were maintained at 33 °C, to compromise between the optimal temperature requirements for fish and mammalian cells.43,44 Data are representative of at least two independent and blind experiments with ⩾30 embryos per group. Experiments with survival rate of control group lower than 80% were discarded.
Male 4–5-week-old athymic nude mice (Balb/c nu/nu n=10 per group) purchased from Charles River (L’Arbresle, France) were housed in ventilated cages under sterile condition and sterile food and water provided ad libitum. As animals were not treated with any drug, randomoization was not necessary after cell inoculation. Inoculation of cells was not done blind. Animal experiment has been approved by the local committee for animal health ethics and research of Leiden University (DEC no. 14226) and carried out in accordance with the European Communities Council Directive 86/609/EEC.
Intracardiac PCa cell injection and whole-body bioluminescent imaging
A single-cell suspension (1 × 105 PC-3M-Pro4Luc2 CRIPTO knockdown or NT control cells per 100 μl PBS) was injected into the left ventricle of anesthetized 5-week-old male nude mice (Balb/c nu/nu). Tumor growth and metastasis formation was monitored weekly by bioluminescent imaging using IVIS100 Imaging System (Caliper LifeSciences, Waltham, MA, USA).12 Analyses of images was performed and quantified with Living Image 4.2 (Caliper LifeSciences).
Five micrometers of formalin-fixed, paraffin-embedded sections were dewaxed and rehydrated using xylene and ethanol, and endogenous peroxidase was blocked for 20 min in 0.3% H2O2 in PBS. Heat-induced antigen retrieval was carried out in TRIS-EDTA buffer (pH=9, 4397-9001; Klinipath, Duiven, The Netherlands) with a pressure cooker (1.2 bar). Antibodies (anti-CRIPTO, no. PBL6900;31 anti-cytokeratin-18, clone DC10; Dako, Santa Clara, CA, USA) were diluted 1:2000 in PBS-BSA 0.1% and incubated overnight at 4 °C. Envision (K500711; Dako) was used to visualize the antibody, followed by counterstaining with hematoxylin.
Clinical prostate cancer bone metastases (10 patients) were collected by the Erasmus MC Tissue Bank under ISO 15189:2007 standard operating procedures and used according to the Human Tissue and Medical Research Code for responsible use (2011). For human callus tissue (source of osteoblast) approval of the Institutional Review Board was obtained and confirmation that the Medical Research Involving Human Subjects Act (WMO) does not apply to the present study was obtained by the local ethics committee since the research was performed on 'waste material' (three patients) collected from Academisch Medisch Centrum (Amsterdam, The Netherlands).
Statistical analysis was performed with GraphPad Prism 6.0 (GraphPad software, La Jolla, CA, USA) using two-tailed unpaired t-test or analysis of variance for comparison between more groups. F-test was used to compare variances and standard deviations between the groups compared to meet the assumption of the statistical test. Data are presented as mean±s.e.m. P-values ⩽0.05 were considered to be statistically significant (*P<0.05, **P<0.01 and ***P<0.001).
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We thank Tim Rodenburg and Janine Melsen (Department of Urology, LUMC) for technical support and Dr Boudewijn PT Kruithof (Department of Molecular Cell Biology, LUMC) for technical support and critical reading of the manuscript, Guido de Roo from the Flow cytometry facility (Department of Hematology, LUMC) and Jan Kroon (Department of Urology, LUMC) for providing experimental metastases. This research received funding from: FP7 Marie Curie ITN, Grant No. 264817—BONE-NET (to GvdP, EZ, ZG); Dutch Cancer Society, Grant No. UL2015-7599 KWF (to MK-dJ, GvdP, PK); Leiden University Fond (to MK-dJ) and Clayton Foundation for Biomedical Research (to PCG).
The authors declare no conflict of interest.
Supplementary Information accompanies this paper on the Oncogene website
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Zoni, E., Chen, L., Karkampouna, S. et al. CRIPTO and its signaling partner GRP78 drive the metastatic phenotype in human osteotropic prostate cancer. Oncogene 36, 4739–4749 (2017). https://doi.org/10.1038/onc.2017.87
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