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Transcriptional intermediary factor 1γ binds to the anaphase-promoting complex/cyclosome and promotes mitosis

Oncogene volume 32, pages 4622–4633 (2013)Cite this article

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Abstract

The anaphase-promoting complex/cyclosome (APC/C) is an ubiquitin ligase that functions during mitosis. Here we identify the transcriptional regulator, transcriptional intermediary factor 1γ, TIF1γ, as an APC/C-interacting protein that regulates APC/C function. TIF1γ is not a substrate for APC/C-dependent ubiquitylation but instead, associates specifically with the APC/C holoenzyme and Cdc20 to affect APC/C activity and progression through mitosis. RNA interference studies indicate that TIF1γ knockdown results in a specific reduction in APC/C ubiquitin ligase activity, the stabilization of APC/C substrates, and an increase in the time taken for cells to progress through mitosis from nuclear envelope breakdown to anaphase. TIF1γ knockdown cells are also characterized by the inappropriate presence of cyclin A at metaphase, and an increase in the number of cells that fail to undergo metaphase-to-anaphase transition. Expression of a small interfering RNA-resistant TIF1γ species relieves the mitotic phenotype imposed by TIF1γ knockdown and allows for mitotic progression. Binding studies indicate that TIF1γ is also a component of the APC/C-mitotic checkpoint complex (MCC), but is not required for MCC dissociation from the APC/C once the spindle assembly checkpoint (SAC) is satisfied. TIF1γ inactivation also results in chromosome misalignment at metaphase and SAC activation; inactivation of the SAC relieves the mitotic block imposed by TIF1γ knockdown. Together these data define novel functions for TIF1γ during mitosis and suggest that a reduction in APC/C ubiquitin ligase activity promotes SAC activation.

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Acknowledgements

We thank Francis Barr, Andrew Fry, Stefano Piccolo, Frank Rauscher III, Hughes de Thé and Hiroyuki Yamano for reagents. This work was funded by CR-UK (C10000/A7542) and The University of Birmingham, College of Medical and Dental Sciences Research Development Fund. JN is supported by The Danish Cancer Society and the Lundbeck Foundation.

Author contributions: GGS and AST initiated the study; GGS, JN and AST designed the study; GGS, KT, AM, NJS, JN and AST performed experiments and analyzed data. RJAG and GSS helped supervise the study. AST wrote the paper.

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Authors and Affiliations

  1. School of Cancer Sciences, College of Medical and Dental Sciences, The University of Birmingham, Edgbaston, Birmingham, UK,

    G G Sedgwick, K Townsend, A Martin, N J Shimwell, R J A Grand, G S Stewart & A S Turnell

  2. NNF Center for Protein Research, The University of Copenhagen, Blegdamsvej, Copenhagen N, Denmark,

    G G Sedgwick & J Nilsson

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  1. G G Sedgwick
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Correspondence to A S Turnell.

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Sedgwick, G., Townsend, K., Martin, A. et al. Transcriptional intermediary factor 1γ binds to the anaphase-promoting complex/cyclosome and promotes mitosis. Oncogene 32, 4622–4633 (2013). https://doi.org/10.1038/onc.2012.501

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