Using genome-wide methylation screening, we identified that paired box gene 5 (PAX5) is involved in human cancer development. However, the function of PAX5 in gastric cancer (GC) development is largely unclear. We analyzed its epigenetic inactivation, biological functions and clinical application in GC. PAX5 was silenced in seven out of eight GC cell lines. A significant downregulation was also detected in paired gastric tumors compared with adjacent non-cancerous tissues. The downregulation of PAX5 was closely linked to the promoter hypermethylation status and could be restored with demethylation treatment. Ectopic expression of PAX5 in silenced GC cell lines (AGS and BGC823) inhibited colony formation and cell viability, arrested cell cycle, induced apoptosis, suppressed cell migration and invasion and repressed tumorigenicity in nude mice. Consistent with the induction of apoptosis by PAX5 in vitro, terminal deoxynucleotidyl transferase-mediated dUTP-digoxigenin nick end labeling (TUNEL) staining showed significantly enhanced apoptotic cells in PAX5-expressed tumors compared with the vector control tumors. On the other hand, knockdown of PAX5 by PAX5–short hairpin RNA increased the cell viability and proliferation. The anti-tumorigenic function of PAX5 was revealed to be mediated by upregulating downstream targets of tumor protein 53 (p53), p21, BCL2-associated X protein, metastasis suppressor 1 and tissue inhibitors of metalloproteinase 1, and downregulating BCL2, cyclin D1, mesenchymal–epithelial transition factor (MET) and matrix metalloproteinase 1. Immunoprecipitation assay demonstrated that PAX5 directly bound to the promoters of p53 and MET. Moreover, PAX5 hypermethylation was detected in 77% (144 of 187) of primary GCs compared with 10.5% (2/19) of normal gastric tissues (P<0.0001). GC patients with PAX5 methylation had a significant poor survival compared with the unmethylated cases as demonstrated by Cox regression model and log-rank test. In conclusion, PAX5 is a novel functional tumor suppressor in gastric carcinogenesis. Detection of methylated PAX5 can be utilized as an independent prognostic factor in GC.
Although the overall incidence of gastric cancer (GC) is declining in almost every country, it is still a serious health problem and remains the second to fourth most common cancer-related death worldwide. There are marked geographical variations, with China being affected most. Although the mechanism leading to cancer development remains elusive, epigenetic inactivation of tumor suppressor genes by promoter methylation is increasingly recognized to have a crucial role in gastric carcinogenesis. The identification of novel genes targeted by promoter hypermethylation may provide insights into the mechanisms for the inactivation of tumor suppressive pathway and is important for identification of tumor marker in GC (Kang et al., 2003a, 2003b; Lee et al., 2004).
Through methylation-sensitive representational difference analysis, we have recently identified paired box gene 5 (PAX5) to be hypermethylated at its promoter in human cancer (Liu et al., 2011). PAX5 was recently characterized as the key nuclear protein in the paired-box-containing family of transcription factors that involved in control of organ development and tissue differentiation (Carotta et al., 2006). PAX5, also known as B-cell-specific activator protein, has an essential role as a transcription factor in early stage of B-cell differentiation, as well as neural development and spermatogenesis (Barberis et al., 1990; Adams et al., 1992; Nutt et al., 1997). PAX5 functions as gene expression activator (Kozmik et al., 1992; Zwollo and Desiderio, 1994) or suppressor (Souabni et al., 2002; Holmes et al., 2006) to control the B-cell commitment (Cobaleda et al., 2007). Previous studies have identified that the PAX5 gene, which is located on chromosome 19p13 (Stapleton et al., 1993; Kovac et al., 2000), is associated frequently with chromosomal translocations (Busslinger et al., 1996). Aberrant PAX5 expression has been reported in several tumors (Baumann Kubetzko et al., 2004; Meza et al., 2006; Mhawech-Fauceglia et al., 2007; Lazzi et al., 2009), thus dysregulated expression of PAX5 is likely to be involved in carcinogenesis and the malignant progression of human cancers. A recent study demonstrated that the lack of expression of PAX5 in lymphoid neoplasms is associated with promoter hypermethylation, and cases with PAX5 silence were characterized by poor clinical outcome (Lazzi et al., 2009). In this study, we discovered that the frequent loss of PAX5 expression in GC is due to promoter methylation. As altered expression of this transcriptional regulator may be the cause of tumor development, we characterized the biological functions of PAX5 in gastric tumorigenicity both in vitro and in vivo using loss or gain of PAX5 function in GC cells. The molecular basis and downstream regulating pathways of PAX5 as a putative tumor suppressor was further generated. The potential clinical application of PAX5 as a novel biomarker in the outcome of GC was also evaluated in 187 primary GCs.
PAX5 is epigenetically suppressed in GC cell lines
PAX5 transcription was silenced or downregulated in 7/8 (87.5%) of GC cell lines, with KatoIII, MKN28, SNU1, SNU16, AGS and BGC823 being silenced and NCI-N87 being downregulated (Figure 1a). In contrast, mRNA expression could be broadly detected in normal human tissues and fetal tissues (Figure 1b). On the other hand, using methylation-specific PCR and bisulfite genomic sequencing (BGS; Figure 1c), full methylation was detected in five GC cell lines (KatoIII, MKN28, SNU1, AGS and BGC823), and partial methylation was found in one GC cell line (NCI-N87) (Figures 1b and c), whereas unmethylated promoter was detected in expressing cell line (MKN45).
To further confirm whether PAX5 expression was repressed by promoter methylation, 5-aza-2′-deoxycytidine (5-Aza) was used to pharmacologically interfere with promoter methylation in methylated cell lines. As shown in Figure 1d, this treatment resulted in the restoration of PAX5 expression in all cell lines examined, conferring that promoter methylation contributes to the epigenetic suppression of PAX5.
PAX5 expression is downregulated in primary GCs
The mRNA expression of PAX5 was evaluated in 18 paired primary GCs using quantitative reverse transcriptase (RT) PCR. PAX5 was significantly downregulated in gastric tumors compared with their adjacent normal tissues (P<0.05; Figure 2).
PAX5 inhibits GC cell growth
The frequent silencing of PAX5 mediated by promoter methylation in GC, but not in normal gastric tissue, suggested that PAX5 may be a candidate tumor suppressor in gastric carcinogenesis. We thus examined the growth inhibitory effect through ectopic expression of PAX5 in AGS and BGC823, which showed no PAX5 expression. Re-expression of PAX5 mRNA and protein in the transient transfected cell lines was evidenced by RT–PCR and western blot (Figure 3a), which caused a significant decrease in cell viability for 52% in AGS (P<0.01) and 59% in BGC823 (P<0.01), compared with vector-transfected control cells (Figure 3b). The suppressive effect on cancer cell growth was further confirmed by colony formation assay in stably transfected cell lines. Colony numbers were significantly reduced to 41% in AGS (P<0.01), and 42% in BGC823 (P<0.01), compared with the control cells (Figure 3c).
PAX5 induces cell apoptosis and causes cell cycle arrest in G0/G1 phase
To determine whether the PAX5-mediated growth inhibition was the result of apoptosis, cell apoptosis was determined by Annexin V–fluorescein isothiocyanate (FITC)/propidium iodide fluorescence-activated cell sorting (FACS) analysis. Our results showed an increase in the numbers of early apoptotic cells (5.60±0.75% vs 2.87±0.38%, P<0.05) in AGS stably transfected with PAX5 than those vector-transfected AGS cells (Figure 3d).
We investigated the effect of PAX5 on cell cycle distribution. FACS analysis of PAX5-transfected AGS revealed a significant decrease in the number of cells in the S phase compared with controls (42.6±3.7% vs 35.0±1.6%, P<0.05), conferring the inhibitory effect of PAX5 on cell proliferation. Concomitant with this inhibition, there was a significant increase in the number of cells accumulating in the G0/G1 phase (49.2±1.5% vs 58.6±2.6%, P<0.01; Figure 3e), thus PAX5 blocks the cell cycle at the G0/G1 checkpoint.
PAX5 inhibits GC cell migration and invasion
To investigate the effect of PAX5 in cancer cell migration, the monolayer wound-healing assay was performed. A significant delay in the closure of the wound gaps in AGS cells transfected with PAX5 as compared with cells transfected with empty vector was observed at both 24 and 48 h (Figure 3f). For the quantitative assessment of cell metastasis and invasiveness, we performed the matrigel invasion assay. The invaded cell number in AGS with PAX5 expression was significantly lower than in control AGS without PAX5 expression (P<0.05; Figure 3g), suggesting that PAX5 inhibits the migration and the invasion of GC cells.
Knockdown of PAX5 promotes cell growth
To further confirm the role of PAX5 in cell growth, the effect of PAX5 was investigated through knockdown PAX5 with short hairpin RNA (shRNA) in a normal immortalized cell line C2C12. PAX5 was significantly knocked down by PAX5–shRNA (Figure 4a). Knockdown of PAX5 markedly enhanced cell viability (P<0.001; Figure 4b) and colony formation (P<0.01; Figure 4c) compared to cells treated with the control shRNA. These data provide evidences that PAX5 functions as a potential tumor suppressor.
PAX5 inhibits tumor growth in nude mice
In light of the observed anti-proliferative and pro-apoptotic effects of PAX5 on the cell lines in vitro, we tested whether PAX5 could alter growth of GC cells in vivo. The tumor growth of BGC823 stably transfected with PAX5 or empty vector (pcDNA3.1) in nude mice was shown in Figure 5a1. The tumor size was significantly lower in PAX5-transfected nude mice as compared with the control mice (P<0.0001; Figure 5a2). Moreover, the mean tumor weight was significantly lesser in PAX5-transfected tumors compared with the control vector-transfected tumors (P<0.05; Figure 5a3). Expression of PAX5 protein in isolated tumor cells transfected with PAX5 was confirmed with PAX5-positive signal in nucleus by immunohistochemistry (Figure 5b).
Terminal deoxynucleotidyl transferase-mediated dUTP-digoxigenin nick end labeling (TUNEL) staining was performed to validate the apoptosis induced by PAX5 in the xenograft tumors (Figure 5c1). Consistent with the result obtained in GC cell lines in vitro, TUNEL-positive cell was higher in PAX5-expressed tumors compared with the vector control tumors (3.62±1.12% vs 1.54±0.71%, P<0.01; Figure 5c2).
Genes modulated by PAX5 are generated
To elucidate the molecular basis by which PAX5 controls GC growth, gene expression profile in PAX5 stably transfected AGS and HCT116 cell lines were analyzed by complementary DNA (cDNA) expression array. The anti-tumorigenesis effect by PAX5 was mediated by regulating important genes involved in apoptosis, cell proliferation and metastasis, as derived from both AGS and HCT116 cell lines (Table 1). PAX5 increased the expression of pro-apoptotic genes including tumor protein 53 (p53), BCL2-associated X protein (BAX) and downregulated anti-apoptotic gene BCL2. PAX5 also enhanced expression of p21 (CDKN1A), a cell cycle regulator, In addition, inhibition of GC cell migration and invasion by PAX5 may result from the enhancement of metastasis suppressor 1 (MTSS1) and tissue inhibitors of metalloproteinase 1 (TIMP1), and depression of mesenchymal–epithelial transition factor (MET) and matrix metalloproteinase 1 (MMP1). Western immunoblot analysis confirmed that p53 and p21 proteins were expressed at higher levels in the PAX5-transfected cells compared with control vector-transfected cells (Figure 6a). PAX5 also enhanced protein expression of cyclin D1 (Figure 6a), a key G1 phase regulator.
PAX5 directly binds to the promoters of p53 and MET
To evaluate whether the observed PAX5-mediated gene expression was associated with direct promoter binding, chromatin immunoprecipitation (ChIP) assay using specific PAX5 antibody was performed in AGS cells, followed by PCR targeting the promoter regions (Figure 6b). ChIP–quantitative PCR assay indicated that PAX5 binds to the promoters of p53 and MET in AGS cells (P<0.01; Figure 6b). However, promoters of MTSS1 and TIMP1 showed no physical interaction with PAX5 protein. This finding indicated that p53 and MET genes are the direct targets of PAX5 in GC cells.
Frequent PAX5 methylation is detected in primary GC patients
PAX5 methylation was evaluated in 187 primary GC tissues and 19 normal gastric biopsy samples by BGS. The methylation level was significantly higher in GC tissues than in normal controls (P<0.0001, Figure 7a), and the methylated PAX5 was detected in 77% (144 of 187) of primary GCs compared with 10.5% (2/19) of normal gastric tissues (P<0.0001). The association between clinicopathological features and PAX5 methylation in human GCs is listed in Supplementary Table 1. There was no correlation between the methylation of PAX5 and clinicopathological features, such as age, gender, Helicobacter pylori (H. pylori) status, histological type, differentiation or pathologic stage.
PAX5 methylation is associated with poor survival of GC patients
The features of GC patients related to the survival status are shown in Table 2. As expected, Tumor-Nodes-Metastases (TNM) stage was a significant prognostic factor (P<0.0001). Moreover, PAX5 methylation was found to be significantly associated with death (P=0.0009). Using univariate Cox regression analysis (Supplementary Table 2), PAX5 methylation was associated with a significantly increased risk of cancer-related death. The relative risk (RR) was 1.963 with 95% confidence interval ranging from 1.127 to 3.418 (P=0.01). After the adjustment for potential confounding factors, multivariate Cox regression analysis showed that PAX5 methylation was a predictor of poorer survival of GC patients (RR, 2.0983; 95% confidence interval, 1.171–3.760); P=0.01; Table 3). As shown in the Kaplan–Meier survival curves, GC patients with PAX5 methylation (median survival of 1.65 years) had poorer survival than others (median survival of 5.20 years; Figure 7b), which showed statistically significant difference based on the log-rank test (P=0.0152, hazard ratio=1.770).
We demonstrated that PAX5 is widely expressed in normal human tissues and fetal tissues, but frequently silenced or downregulated in GC cell lines. Downregulation of PAX5 was also observed in primary gastric tumors compared with their adjacent non-cancerous tissues, suggesting PAX5 may be important in gastric carcinogenesis. The silencing or downregulation of PAX5 was revealed to be related closely with promoter hypermethylation, as demonstrated by methylation-specific PCR and confirmed by BGS analysis. This was further validated by demethylation treatment with the demethylating reagent 5-Aza; restored expression of PAX5 in silenced cancer cells was obtained following treatment. These results indicated that promoter hypermethylation of PAX5 directly mediated the PAX5 transcriptional silence. One GC cell line SNU16 showed no PAX5 expression and no promoter methylation, suggesting that other transcription-regulating mechanisms, such as histone modification (Jones et al., 1998) or upstream transcriptional regulation (Lee et al., 2008), might also contribute to PAX5 gene silencing. Collectively, our data revealed that the epigenetic regulation mechanism (promoter methylation) on the PAX5 gene has an important role in its transcriptional silence, which may contribute to the development of GC.
The biological function of PAX5 in human GC was further investigated by gain- and loss-PAX5 function assays. Ectopic expression of PAX5 in silenced cancer cell cells significantly inhibited cell viability and colony formation ability. Cell cycle distribution analysis by flow cytometry revealed a significant decrease in cell proliferation and a proportionate increase of cells in G0/G1 phase following PAX5 transfection. Concomitantly, ectopic expression of PAX5 significantly induced cell apoptosis, decreased cell migration and cell invasion ability in GC cell lines. Conversely, knockdown of PAX5 by shRNA significantly promoted cell growth. Having observed the substantial suppression of GC cell growth by PAX5 in vitro, we studied tumor suppressive effect of PAX5 against gastric tumor formation in vivo. Our result showed that the tumor growth was significantly retarded in nude mice inoculated with BGC823/PAX5 compared with those inoculated with BCG823/vector subcutaneously. Together, the consistent results observed in vitro and in vivo indicated for the first time that PAX5 functions as a tumor suppressor in gastric carcinogenesis. This understanding of the anti-tumor effect PAX5 in GC suggests that restoration of the function of PAX5 could halt or reverse the abnormalities, thus having a potential therapeutic effect.
We further uncovered the molecular basis of PAX5 exerting the tumor suppressor property in GC using cDNA microarray, western blot and ChIP assays (Figure 6c). The enhanced apoptosis ability by PAX5 was discovered to be mediated at least by the direct upregulation of pro-apoptotic p53 through binding to p53 promoter and subsequent modulation of p53 downstream targets BAX and BCL2 (Figure 6c). P53 is known as a tumor suppressor gene that is capable BCL2 of inducing transcriptional regulation of other anti-tumorigenic molecules (Levine, 1989; Kishimoto et al., 1992; Malkin et al., 1994; Stuart et al., 1995). Induction of p53 had been reported to downregulate BCL2 in breast cancer (Haldar et al., 1994) and upregulate BAX in lung cancer (Miyashita and Reed, 1995), two important mediators in the process of program cell death. BAX induces apoptotic cell death by cytokine deprivation and represses activity of BCL2, an apoptotic blocker (Hockenbery et al., 1991; Oltvai et al., 1993; Ruvolo et al., 2001). Therefore, upregulation of p53 and its downstream targets induced by PAX5 could explain the effect of PAX5 in inducing cell apoptosis.
On the basis of the cDNA microarray and western blot analyses, the cell cycle arrest in G0/G1 phase caused by PAX5 was mostly attributable to the upregulation of p21 and downregulation of cyclin D1 (Figure 6c). It has been reported that p53 can directly enhance the transcription of p21 (el-Deiry et al., 1993). P21 is a potent inhibitor of cyclin D/Cdk4 and cyclin E/Cdk2, whose kinases govern cell–cycle progression at the restriction and late transition points of G1 (He et al., 2005). In addition, inhibition of cyclin D1, a key regulatory subunit of CDK4, could inhibit the cell cycle entry into S phase (Quelle et al., 1993; Musgrove et al., 1994). P53 was reported to cause G1 cell cycle arrest by indirectly suppressing cyclin D1 transcription (el-Deiry et al., 1993; Rocha et al., 2003).
Ectopic expression of PAX5 suppressed the cell migration, as demonstrated by the wound healing assay. This effect was further confirmed by matrigel invasion analysis, suggesting that PAX5 could reduce metastasis and invasiveness of GC cells. Cell migration and invasion are critical events during the cancer progression to metastasis. The anti-migration/invasion effect caused by PAX5 in GC cells was contributed to the upregulation of MTSS1 and TIMP1 and downregulation of MMP1 (Figure 6c). MTSS1 was recently identified as a putative metastasis suppressor, with an important role in inhibiting the metastasis of bladder cancer (Lee et al., 2002; Wang et al., 2007) and breast cancer (Parr and Jiang, 2009). MMP1 has an important function in cancer progression by promoting invasion and metastasis in prostate and lung cancer (Chambers and Matrisian, 1997; Sauter et al., 2008). TIMP1 is an inhibitor of the known MMPs including MMP1 (Visse and Nagase, 2003). Collectively, induction of MTSS1 and TIMP1 and suppression of MMP1 by PAX5 could explain the ability of PAX5 in governing the metastatic nature of GC.
The anti-tumorigenic function of PAX5 was also related to the direct downregulation of MET, a well-known proto-oncogene. MET encodes a transmembrane receptor tyrosine kinase (Bottaro et al., 1991). The latter can be mutated or overexpressed in a number of epithelial human cancers, including GC, which triggers cell growth, motility and invasiveness (Giordano et al., 1993; Benvenuti and Comoglio, 2007; Kanteti et al., 2009). We found that PAX5 downregulated MET expression by directly binding to the promoter of MET, as evidenced by cDNA expression array and ChIP–quantitative PCR assays. Thus, downregulation of MET by PAX5 also had a role in the anti-tumorigenic/metastatic properties of PAX5.
To investigate the clinical application of PAX5 in gastric tumorigenesis in vivo, we examined the promoter methylation of PAX5 by BGS in 187 primary GCs and 19 normal controls. PAX5 gene promoter was found to be commonly methylated in GCs (77%, 144/187) as compared with normal controls (10.5%, 2/19; P<0.0001). Recognizing the tumor suppressor effect of PAX5, the inactivation of this gene by promoter methylation would favor tumor progression and a worse outcome. In this regard, the clinical significance of PAX5 promoter methylation and its associations with patient outcome were evaluated in 187 primary GC patients. Our results indicated that PAX5 methylation was significantly associated with a shorter survival independent of patient clinicopathological characteristics (RR, 2.10; 95% confidence interval, 1.171–3.760; P=0.01). Our data support an adverse effect of PAX5 promoter methylation on survival of GC patients. PAX5 methylation could be regarded as a valuable new prognostic factor for GC.
In conclusion, we have identified a novel functional tumor suppressor gene PAX5 inactivated by promoter methylation in GC, with important roles in suppressing cell proliferation, inducing apoptosis and inhibiting cell invasion. PAX5 induced cancer cell apoptosis through direct upregulation of p53 and mediating its downstream targets (BAX and BCL2). PAX5 suppressed cell proliferation through upregulation of p21 and downregulation of cyclin D1. PAX5 inhibited cell invasion/metastasis by inducing MTSS1 and TIMP1 and inhibiting MMP1. The anti-tumorigenic function of PAX5 was also mediated by directly downregulating oncogene MET. PAX5 methylation could serve as a putative epigenetic biomarker to predict outcome for GC patients.
Materials and methods
GC cell lines (AGS, Kato III, MKN28, MKN45, N87, SNU1 and SNU16) and immortalized mouse myoblast cell line (C2C12) were purchased from American Type Culture Collection (Manassas, VA, USA). GC cell line BGC823 was kindly provided from Beijing Oncology Hospital, Beijing, China.
Patients and primary human samples
Paired biopsy samples from primary tumor and adjacent non-tumor sites were obtained from 18 GC patients during endoscopy before any therapeutic intervention. Biopsy samples from the adjacent non-tumor areas were subsequently verified by histology to be free of tumor infiltration. The biopsies were snap frozen in liquid nitrogen and stored −80 °C for molecular analyses. The remaining tissue specimens were fixed in 10% formalin and embedded in paraffin for routine histological examination.
A total of 187 primary GC tissues were collected from patients at the time of operation. This included 124 men and 63 women, with average age of 57.1±12.8 years. Tumor was staged according to the Tumor, Nodes, and Metastases (TNM) staging system. Other clinicopathological features, such as H. pylori infection and tumor differentiation, were also recorded. In addition, 19 age-matched subjects (average age 51.9±17.2 years) with normal upper gastroscopy were recruited as control. GC patients were being regularly followed up, as the time of diagnosis and the follow-up duration was 0–97.6 months. In total, 91 (48.7 %) patients died in the follow-up period. Informed consent was given by all the patients and controls, and the study protocol was approved by the Clinical Research Ethics Committee of the Chinese University of Hong Kong.
Semiquantitative RT–PCR and real-time quantitative PCR analyses
Total RNA was extracted from tissues and cell pellets using QIAzol reagent (Qiagen, Valencia, CA, USA). cDNA was synthesized from 2 μg total RNA using Transcriptor Reverse Transcriptase (Roche Applied Sciences, Indianapolis, IN, USA). Semiquantitative RT–PCR and real-time quantitative PCR analyses were performed using primers listed in Supplementary Table 3.
Western blot analysis
Total protein was extracted, and protein concentration was measured by the DC protein assay method of Bradford (Bio-Rad, Hercules, CA, USA). A quantity of 40 μg of protein from each sample was separated by 10% SDS–polyacrylamide gel electrophoresis gel. The protein was transferred to an equilibrated polyvinylidene difluoride membrane (Amersham Biosciences, Buckinghamshire, UK) and then incubated in specific primary antibody at 4 °C overnight. After incubation with the secondary antibody, the proteins were detected by enhanced chemiluminescence (Amersham Corporation, Arlington Heights, IL, USA).
Demethylation treatment using 5-Aza
Cells were seeded at a density of 1 × 105 cells/ml in 100-mm dishes and grown for 24 h. Cells were then treated with 2 μM 5-Aza (Sigma-Aldrich, St Louis, MO, USA) for 5 days. 5-Aza was replenished every day.
DNA extraction and methylation-specific PCR
Genomic DNA from cancer cell lines and tissue samples were isolated by using DNA mini kit (Qiagen, Hilden, Germany). One microgram of DNA was modified by sodium metabisulfite as described previously (Tao et al., 2002). The bisulfite-modified DNA was amplified by methylation-specific PCR using primer pairs (Supplementary Table 3) that specifically amplify either methylated or unmethylated sequences of the PAX5 gene.
Bisulfite genomic sequencing
A total volume of 2 μl of bisulfite-treated DNA was amplified by primers of BGS (Supplementary Table 3). Sequencing was performed using the BigDye Terminator Cycle Sequencing kit version 1.0 (Applied Biosystems, Foster City, CA, USA). Sequences were analyzed by using SeqScape software (Applied Biosystems) and Bioedit (http://www.mbio.ncsu.edu/BioEdit/bioedit.html). Methylation percentage of each CpG site was calculated according to the formula: methylation%=HC/(HC+HT) × 100% (HC=height of peak C and HT=height of peak T).
Construction of PAX5 expression vector and PAX5 shRNA vector
The expression vector (pcDNA3.1–PAX5) encoding the full-length open reading frame of human PAX5 gene was constructed by PCR cloning. Briefly, total RNA from human stomach (Ambion, Austin, TX, USA) was reverse transcribed into cDNA. Sequence corresponding to the open reading frame of PAX5 was amplified by PCR and verified by DNA sequencing. PCR-amplified insert was subcloned into the pCDNA3.1 TOPO TA expression vector (Invitrogen, Carlsbad, CA, USA). The shRNA vector pGFP-V-RS-shPAX5 and its control vector were purchased from OriGene (Rockville, MD, USA).
Colony formation assay
Cancer cells (AGS, HTC116 and BGC823; 2 × 105/well) were plated in six-well plates and transfected with pcDNA3.1–PAX5 or empty vector using Lipofectamine 2000 (Invitrogen). Two days after transfection, cells were subsequently split at 1:20 ratio in six-well plates with 0.5 mg/ml neomycin (G418; Invitrogen). On the other hand, C2C12 cells (5 × 105/well) were plated in a 6-well plate and transfected with 2 μg/ml pGFP-V-RS-shPAX5 or control shRNA vector using Lipofectamine 2000. Two days after transfection, cells were subsequently split at 1:20 ratio in 6-well plates with 0.5 μg/ml puromycin (Invitrogen). After 14–18 days of selection to establish stable clones, cells were fixed with 70% ethanol and stained with crystal violet solution. Colony with more than 50 cells/colony was counted. The experiment was conducted in three independent triplicates.
Cell viability assay
Cell viability was determined by 3-(4, 5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium assay using CellTiter 96 AQueous One Solution Cell Proliferation Assay kit (Promega, Madison, WI, USA).
Cell migration assay
Cell migration was assessed using a scratch wound assay. Briefly, pcDNA3.1–PAX5 or empty vector stably transfected AGS cells were cultured in six-well plates (5 × 105 cells/well). When the cells grew up to 90% confluence, three scratch wounds across each well were made using a P-200 pipette tip(Axygen, Union City, CA, USA). Images of the wound closure areas were taken at 0, 24 and 48 h.
Matrigel invasion assay was performed on pcDNA3.1–PAX5 or empty vector stably transfected AGS using the 24-well matrigel-biocoated invasion chamber (BD Biosciences, Bedford, MA, USA) as described previously (Ying et al., 2006).
The stably transfected AGS cells with pcDNA3.1–PAX5-expressing or pcDNA3.1 empty vector were fixed in 70% ethanol and stained with 50 μg/ml propidium iodide (BD Pharmingen, San Jose, CA, USA). The cells were then sorted by FACSCalibur (BD Biosciences, Franklin Lakes, NJ, USA) and cell cycle profiles were analyzed by ModFit 3.0 software (Verity Software House, Topsham, ME, USA). Apoptosis was determined by dual staining with Annexin V-FITC (Invitrogen) and propidium iodide. The Annexin V-positive cells were counted as apoptotic cells.
In vivo tumorigenicity
BGC823 cells (1 × 106 cells in 0.1 ml phosphate-buffered saline) transfected with PAX5 or pcDNA3.1 were injected subcutaneously into the dorsal left flank of 5-week-old male Balb/c nude mice as described previously (Yu et al., 2010). All experimental procedures were approved by the Animal Ethics Committee of the Chinese University of Hong Kong.
Immunostaining and in situ DNA nick end labeling
Immunohistochemical staining was performed using PAX5-specific antibody as described previously (Yu et al., 2010). TUNEL assay was performed with Dead End TM Colorimetric TUNEL System (Promega). The apoptotic cell ratio was calculated as percentage of positive cells in at least 1000 cells.
cDNA expression array
Gene expression profiles in both AGS and HCT116 stably transfected with pcDNA3.1–PAX5 or pcDNA3.1 empty vector were analyzed by the Human Cancer PathwayFinder RT2 Profiler PCR Array (SABiosciences, Frederick, MD, USA; http://www.sabiosciences.com) according to the protocol. Genes with fold changes more than or less than 1.5 were considered to be of biological significance.
ChIP analysis was performed by using Red ChIP Kit (Diagenode, Liège, Belgium). The immunoprecipitated and input DNA in AGS/vector and AGS/PAX5 cells was used as a template for quantitative PCR analysis using the primers listed in Supplementary Table 3.
Mann–Whitney U test was performed to compare the pathological variables of the two sample groups in the functional assay. The difference in tumor growth rate between the two groups of nude mice was determined by repeated measures analysis of variance. The Fisher's exact test was used for analysis of patient features. RRs of death associated with PAX5 methylation status and other predictors were estimated by univariate Cox proportional hazards model. Multivariate Cox models were also constructed to estimate the RR for PAX5 methylation. Kaplan–Meier survival curve and log-rank test were used to evaluate overall survival data corresponding to PAX5 methylation status. Data were considered statistically significant when P-value is <0.05.
BCL2-associated X protein
bisulfite genomic sequencing
- H. pylori :
mesenchymal epithelial transition factor
matrix metalloproteinase 1
methylation specific PCR
metastasis suppressor 1
open reading frame
tumor protein 53
- PAX5 :
paired box gene 5
phosphate buffered saline
quantitative polymerase chain reaction
tissue inhibitors of metalloproteinase 1
terminal deoxynucleotidyl transferase-mediated dUTP-digoxigenin nick end labelling
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This study was supported by research grants of National Basic Research Program of China (973 Program, 2010CB529305), Hong Kong General Research Fund (473008), RFCID (11100022, 10090942), CUHK Group Research Scheme (3110043) and Scheme C.
The authors declare no conflict of interest.
Supplementary Information accompanies the paper on the Oncogene website
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Li, X., Cheung, K., Ma, X. et al. Epigenetic inactivation of paired box gene 5, a novel tumor suppressor gene, through direct upregulation of p53 is associated with prognosis in gastric cancer patients. Oncogene 31, 3419–3430 (2012). https://doi.org/10.1038/onc.2011.511
- paired box gene 5
- gastric cancer
- tumor suppressor gene
- epigenetic alteration
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