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Phosphoproteomic analysis of Syk kinase signaling in human cancer cells reveals its role in cell–cell adhesion

Oncogene volume 28, pages 2337–2347 (2009)Cite this article

Abstract

The spleen tyrosine kinase Syk has predominantly been studied in hematopoietic cells in which it is involved in immunoreceptor-mediated signaling. Recently, Syk expression was evidenced in numerous nonhematopoietic cells and shown to be involved in tumor formation and progression. The Syk downstream signaling effectors in nonhematopoietic cells remain, however, to be uncovered, and were investigated using MS-based quantitative phosphoproteomics. Two strategies, based on the inhibition of the Syk catalytic activity and on the loss of Syk expression were employed to identify phosphotyrosine-dependent complexes. Quantitative measurements were obtained on 350 proteins purified with phosphotyrosine affinity columns using the SILAC method. Forty-one proteins are dependent on both Syk expression and catalytic activity and were selected as signaling effectors. They are involved in a variety of biological processes such as signal transduction, cell–cell adhesion and cell polarization. We investigated the functional involvement of Syk in cell–cell adhesion and demonstrated the phosphorylation of E-cadherin and α-catenin. In addition, Syk is localized at cell–cell contacts, and Syk-mediated phosphorylation of E-cadherin seems to be important for the proper localization of p120-catenin at adherens junctions. Identification of the biochemical pathways regulated by Syk in human cancer cells will help to uncover its role in tumor formation and progression.

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Abbreviations

Pic:

piceatannol

PV:

pervanadate

SILAC:

Stable Isotope Labeling with Amino acids in Cell culture

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Acknowledgements

We thank A Morel and M Saen for skilled technical assistance; F Raynaud and C Roy for valuable technical advice; G Freiss for IRS-1 and S Baghdiguian for p65-NF-κB antibodies; M Duñach and A Garcìa de Herreros for GST constructs; Montpellier RIO Imaging platform for image analysis. MS experiments were performed at the Functional Proteomics Platform located at the IGF. This study was supported by the Centre National de la Recherche Scientifique, the Institut National du Cancer (no. PL06-111 to PJ Coopman) and the Ligue Nationale contre le Cancer (LNCC; Equipe Labellisée Ligue 2007 to PJ Coopman). RM Larive is a recipient of a PhD fellowship funded by the LNCC and the Association pour la Recherche sur le Cancer (ARC).

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Author notes

  1. P J Coopman and N Bettache: These authors contributed equally to this work.

Authors and Affiliations

  1. Centre de Recherche de Biochimie Macromoléculaire, CNRS UMR 5237, IFR 122, Equipe Labellisée 2007 Ligue Nationale contre le Cancer, Universités Montpellier 1 and 2, Montpellier Cedex 5, France

    R M Larive, G Mascré, A Sahuquet, P H Mangeat, P J Coopman & N Bettache

  2. Institut de Génomique Fonctionnelle, CNRS UMR 5203, INSERM U661, Universités Montpellier 1 and 2, Montpellier Cedex 5, France

    S Urbach, J Poncet & P Jouin

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  1. R M Larive
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  2. S Urbach
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Correspondence to N Bettache.

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Supplementary Information accompanies the paper on the Oncogene website (http://www.nature.com/onc)

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Larive, R., Urbach, S., Poncet, J. et al. Phosphoproteomic analysis of Syk kinase signaling in human cancer cells reveals its role in cell–cell adhesion. Oncogene 28, 2337–2347 (2009). https://doi.org/10.1038/onc.2009.99

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