Abstract
Menin upregulates transcription of cell-cycle inhibitors to suppress endocrine tumors, but it is poorly understood how menin suppresses non-endocrine tumors such as lung cancer. Here, we show that menin inhibits proliferation of human lung cancer cells and growth of lung cancer in mice. The menin-mediated tumor suppression requires repression of growth factor pleiotrophin (PTN), which binds to its cell surface receptor, anaplastic lymphoma kinase (ALK) that is activated in certain lung adenocarcinomas. Menin represses PTN transcription and PTN-induced proliferation of human lung cancer cells, and menin expression is substantially reduced in primary human lung adenocarcinomas. Notably, menin binds the PTN locus and enhances Polycomb gene Enhancer of Zeste homolog 2 (EZH2)-mediated histone H3 lysine 27 trimethylation (H3K27m3), a negative mark for gene transcription but does not affect histone H3K4 methylation that is usually upregulated by menin in endocrine cells. Together, our findings indicate that menin suppresses lung cancer partly through increasing Polycomb gene-mediated H3K27 methylation and repressing PTN transcription, unraveling a novel, epigenetically regulated PTN–ALK signaling pathway in suppressing lung cancer.
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Acknowledgements
This work is in partly supported by NFSC Grants (No 30701003, GH Jin), the Natural Science Foundation of Fujian Province of China (No C0710044, GH Jin), National Cancer Institute Short-Term Scientist Exchange Program (GH Jin), and National Cancer Institute Grants (R01CA100912 and R01CA113962, XH). We appreciate the valuable comments from other members of our laboratories.
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Gao, SB., Feng, ZJ., Xu, B. et al. Suppression of lung adenocarcinoma through menin and polycomb gene-mediated repression of growth factor pleiotrophin. Oncogene 28, 4095–4104 (2009). https://doi.org/10.1038/onc.2009.273
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DOI: https://doi.org/10.1038/onc.2009.273
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