Brief Communication | Published:

A closed conformation for the Pol λ catalytic cycle

Nature Structural & Molecular Biology volume 12, pages 9798 (2005) | Download Citation

Subjects

Abstract

Pol λ is a family X member believed to fill short gaps during DNA repair. Here we report crystal structures of Pol λ representing three steps in filling a single-nucleotide gap. These structures indicate that, unlike other DNA polymerases, Pol λ does not undergo large subdomain movements during catalysis, and they provide a clear characterization of the geometry and stereochemistry of the in-line nucleotidyl transfer reaction.

Access optionsAccess options

Rent or Buy article

Get time limited or full article access on ReadCube.

from$8.99

All prices are NET prices.

Accessions

References

  1. 1.

    & Functions of DNA polymerases. In DNA Repair and Replication, Advances in Protein Chemistry Vol. 69 (ed. Yang, W.) 137–165 (Elsevier, San Diego, 2004).

  2. 2.

    , et al. Biochemistry 36, 11205–11215 (1997).

  3. 3.

    & Curr. Opin. Struct. Biol. 8, 704–712 (1998).

  4. 4.

    et al. Proc. Natl. Acad. Sci. USA 100, 3895–3900 (2003).

  5. 5.

    , et al. EMBO J. 17, 7514–7525 (1998).

  6. 6.

    & Annu. Rev. Biochem. 69, 497–529 (2000).

  7. 7.

    et al. J. Mol. Biol. 301, 851–867 (2000).

  8. 8.

    et al. J. Biol. Chem. 279, 805–811 (2004).

  9. 9.

    et al. J. Biol. Chem. 276, 34659–34663 (2001).

  10. 10.

    et al. Mol. Cell 13, 561–572 (2004).

  11. 11.

    et al. J. Biol. Chem. 275, 28033–28038 (2000).

  12. 12.

    & Curr. Opin. Struct. Biol. 8, 54–63 (1998).

  13. 13.

    & Biochemistry 18, 450–454 (1979).

Download references

Acknowledgements

The authors thank W. Beard, L. Pedersen and J. Boyington for critical review of the manuscript. Use of the Advanced Photon Source was supported by the US Department of Energy, Office of Science, Office of Basic Energy Sciences, under contract no. W-31-109-Eng-38.

Author information

Affiliations

  1. Laboratory of Structural Biology, National Institute of Environmental Health Sciences, National Institutes of Health, Department of Health and Human Services, Research Triangle Park, North Carolina 27709, USA.

    • Miguel Garcia-Diaz
    • , Katarzyna Bebenek
    • , Joseph M Krahn
    • , Thomas A Kunkel
    •  & Lars C Pedersen
  2. Laboratory of Molecular Genetics, National Institute of Environmental Health Sciences, National Institutes of Health, Department of Health and Human Services, Research Triangle Park, North Carolina 27709, USA.

    • Miguel Garcia-Diaz
    • , Katarzyna Bebenek
    •  & Thomas A Kunkel

Authors

  1. Search for Miguel Garcia-Diaz in:

  2. Search for Katarzyna Bebenek in:

  3. Search for Joseph M Krahn in:

  4. Search for Thomas A Kunkel in:

  5. Search for Lars C Pedersen in:

Competing interests

The authors declare no competing financial interests.

Corresponding author

Correspondence to Thomas A Kunkel.

Supplementary information

PDF files

  1. 1.

    Supplementary Fig. 1

    Superimposition of Pol γ complexes

  2. 2.

    Supplementary Fig. 2

    Minor groove interactions in the active site.

  3. 3.

    Supplementary Fig. 3

    Coordination of the ddTTP with the catalytic metal.

  4. 4.

    Supplementary Table 1

    Crystallographic data statistics.

  5. 5.

    Supplementary Methods

About this article

Publication history

Received

Accepted

Published

DOI

https://doi.org/10.1038/nsmb876

Further reading