Yeast Jhd2p is a histone H3 Lys4 trimethyl demethylase

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  • An Erratum to this article was published on 01 April 2007

Abstract

Histone methylation is important in regulating chromatin structure and function. In budding yeast, methylation of histone H3 at Lys4 (H3-K4) is associated with active transcription and is enriched at the 5′ regions of transcribed genes. Here we identify a novel budding yeast JmjC domain–containing H3-K4 demethylase, Jhd2p, that antagonizes the trimethyl modification state and contributes to regulation of telomeric silencing.

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Figure 1: Budding yeast Yjr119Cp (renamed Jhd2p) is an H3-K4 demethylase.
Figure 2: Jhd2 antagonizes H3-K4me3 methylation and regulates telomeric silencing.

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Acknowledgements

We thank J. Lieb (University of North Carolina, Chapel Hill), H. Dolhman (University of North Carolina, Chapel Hill) and C. Brandl (University of Western Ontario) for providing plasmids, B. Strahl (University of North Carolina, Chapel Hill) for providing the BY4741, set2Δ, spt4Δ, rtf1Δ, snf2Δ, spt7Δ, htz1Δ and sir2Δ strains, and E. Turnbull for critical reading of the manuscript. This work was supported by US National Institutes of Health grant GM68804 (to Y.Z.). Y.Z. is an Investigator of the Howard Hughes Medical Institute. R.J.K. is supported by the Canadian Institutes of Health Research.

Author information

G.L., R.J.K., K.E.G. and Y.Z. designed the experiments. G.L., R.J.K. and K.E.G. carried out the experiments. R.J.K. and Y.Z. wrote the manuscript.

Correspondence to Yi Zhang.

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The authors declare no competing financial interests.

Supplementary information

Supplementary Table 1

Phenotype analysis in the jhd2Δ strain. (PDF 101 kb)

Supplementary Methods (PDF 100 kb)

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Liang, G., Klose, R., Gardner, K. et al. Yeast Jhd2p is a histone H3 Lys4 trimethyl demethylase. Nat Struct Mol Biol 14, 243–245 (2007) doi:10.1038/nsmb1204

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