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Tracking rates of transcription and splicing in vivo

A relatively simple but powerful method to measure RNA polymerase II transcription elongation as well as co-transcriptional RNA splicing rates at many genes in vivo is described in this issue. The results demonstrate a rather uniform, and high, elongation rate at large human genes and co-transcriptional pre-mRNA splicing of both U2- and U12-dependent primary transcripts.

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Figure 1: Stepwise depiction of method used by Singh and Padgett to measure the elongation rate and splicing kinetics at native genes in vivo.

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Ardehali, M., Lis, J. Tracking rates of transcription and splicing in vivo. Nat Struct Mol Biol 16, 1123–1124 (2009). https://doi.org/10.1038/nsmb1109-1123

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