Abstract
The highly conserved ribonuclease RNase Z catalyzes the endonucleolytic removal of the 3′ extension of the majority of tRNA precursors. Here we present the structure of the complex between Bacillus subtilis RNase Z and tRNAThr, the first structure of a ribonucleolytic processing enzyme bound to tRNA. Binding of tRNA to RNase Z causes conformational changes in both partners to promote reorganization of the catalytic site and tRNA cleavage.
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Acknowledgements
We thank F.A. Wollman, D. Drapier, J.L. Popot, G. Hervé, D. Picot, T. Bizebard, F. Dardel, M. Springer, Y. Timsit, R. Giégé and V. Arluison for their contributions and L. Jacquamet at the European Synchrotron Radiation Facility. Funding was from the CNRS (UPR 9073), Université de Paris 7, the Association pour la Recherche sur le Cancer (grant 3506), ACI Jeunes Chercheurs and the Conseil Régionale de l'Ile de France.
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Supplementary information
Supplementary Fig. 1
Cleavage of tRNA reisolated from crystals (PDF 428 kb)
Supplementary Fig. 2
Crystal contacts between tRNA and RNase Z (PDF 68 kb)
Supplementary Fig. 3
Cleavage of tRNA precursor by RNase Z mutants (PDF 341 kb)
Supplementary Fig. 4
Conformational changes in RNase Z upon tRNA binding (PDF 300 kb)
Supplementary Table 1
Data collection and refinement statistics (PDF 61 kb)
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Li de la Sierra-Gallay, I., Mathy, N., Pellegrini, O. et al. Structure of the ubiquitous 3′ processing enzyme RNase Z bound to transfer RNA. Nat Struct Mol Biol 13, 376–377 (2006). https://doi.org/10.1038/nsmb1066
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DOI: https://doi.org/10.1038/nsmb1066
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