Easing selenocysteine into proteins

Article metrics

Selenocysteinyl-tRNASec is used by many organisms from all three domains of life to incorporate selenocysteine (Sec) site-specifically into certain proteins. Two recent reports have identified a new Sec synthase that catalyzes the last step in the generation of this aminoacyl-tRNA in eukarya and archaea.

Access options

Rent or Buy article

Get time limited or full article access on ReadCube.

from$8.99

All prices are NET prices.

Figure 1: Selenocysteine can be incorporated into specific proteins via read-through of an internal, in-frame UGA codon.
Figure 2: The biosynthesis of Sec-tRNASec in bacteria (top pathway) and in archaea and eukarya (bottom pathway).

References

  1. 1

    Bock, A. et al. Mol. Microbiol. 5, 515–520 (1991).

  2. 2

    Boyington, J.C., Gladyshev, V.N., Khangulov, S.V., Stadtman, T.C. & Sun, P.D. Science 275, 1305–1308 (1997).

  3. 3

    Raaijmakers, H.C. & Romao, M.J. J. Biol. Inorg. Chem. 11, 849–854 (2006).

  4. 4

    Bock, A., Thanbichler, M., Rother, M. & Resch, A. in Aminoacyl-tRNA Synthetases (eds. Ibba, M., Francklyn, C.S. & Cusack, S.) 320–327 (Landes Biosciences, Georgetown, Texas, USA, 2005).

  5. 5

    Xu, X.M. et al. PLoS Biol. 5, e4 (2006).

  6. 6

    Yuan, J. et al. Proc. Natl. Acad. Sci. USA 103, 18923–18927 (2006).

  7. 7

    Leinfelder, W., Zehelein, E., Mandrand-Berthelot, M.A. & Bock, A. Nature 331, 723–725 (1988).

  8. 8

    Forchhammer, K., Leinfelder, W., Boesmiller, K., Veprek, B. & Bock, A. J. Biol. Chem. 266, 6318–6323 (1991).

  9. 9

    Bilokapic, S., Korencic, D., Soll, D. & Weygand-Durasevic, I. Eur. J. Biochem. 271, 694–702 (2004).

  10. 10

    Wu, X.Q. & Gross, H.J. Nucleic Acids Res. 21, 5589–5594 (1993).

  11. 11

    Kaiser, J.T. et al. Biochemistry 44, 13315–13327 (2005).

  12. 12

    Carlson, B.A. et al. Proc. Natl. Acad. Sci. USA 101, 12848–12853 (2004).

  13. 13

    Sauerwald, A. et al. Science 307, 1969–1972 (2005).

  14. 14

    Gelpi, C., Sontheimer, E.J. & Rodriguez-Sanchez, J.L. Proc. Natl. Acad. Sci. USA 89, 9739–9743 (1992).

  15. 15

    Kernebeck, T., Lohse, A.W. & Grotzinger, J. Hepatology 34, 230–233 (2001).

  16. 16

    Leinfelder, W., Forchhammer, K., Veprek, B., Zehelein, E. & Bock, A. Proc. Natl. Acad. Sci. USA 87, 543–547 (1990).

  17. 17

    Forchhammer, K. & Bock, A. J. Biol. Chem. 266, 6324–6328 (1991).

  18. 18

    Feng, L. et al. in Aminoacyl-tRNA Synthetases (eds. Ibba, M., Francklyn, C.S. & Cusack, S.) 314–319 (Landes Biosciences, Georgetown, Texas, USA, 2005).

  19. 19

    Krzycki, J.A. Curr. Opin. Microbiol. 8, 706–712 (2005).

Download references

Author information

Ethics declarations

Competing interests

The author declares no competing financial interests.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Hendrickson, T. Easing selenocysteine into proteins. Nat Struct Mol Biol 14, 100–101 (2007) doi:10.1038/nsmb0207-100

Download citation

Further reading