Pluripotent cells have been derived from the inner cell mass of mouse embryos and also from primordial germ cells. A new study shows that cell lines of both origins exhibit highly similar transcriptomes and surprisingly low DNA methylation levels when maintained in culture conditions that support naive pluripotency. These 2i conditions thus provide a closer approximation of in vivo development and new insights into the regulation of DNA methylation.
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Katie Vicari
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Bock, C., Wutz, A. DNA methylation: a matter of culture. Nat Struct Mol Biol 20, 249–251 (2013). https://doi.org/10.1038/nsmb.2531
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DOI: https://doi.org/10.1038/nsmb.2531
