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Nonsense-mediated mRNA decapping occurs on polyribosomes in Saccharomyces cerevisiae

Nature Structural & Molecular Biology volume 17, pages 244–247 (2010)Cite this article

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Abstract

Nonsense-mediated decay (NMD) degrades mRNA containing premature translation termination codons. In yeast, NMD substrates are decapped and digested exonucleolytically from the 5′ end. Despite the requirement for translation in recognition, degradation of nonsense-containing mRNA is considered to occur in ribosome-free cytoplasmic P bodies. We show decapped nonsense-containing mRNA associate with polyribosomes, indicating that recognition and degradation are tightly coupled and that polyribosomes are major sites for degradation of aberrant mRNAs.

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Figure 1: NMD substrates are bound by polyribosomes when mRNA decapping is inhibited.
Figure 2: The association of decapped NMD substrates with polyribosomes.
Figure 3: Decapped products of nonsense-containing mRNA co-purify with ribosomes.

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References

  1. Isken, O. & Maquat, L.E. Genes Dev. 21, 1833–1856 (2007).

    Article  CAS  Google Scholar 

  2. Behm-Ansmant, I. et al. FEBS Lett. 581, 2845–2853 (2007).

    Article  CAS  Google Scholar 

  3. Amrani, N., Sachs, M.S. & Jacobson, A. Nat. Rev. Mol. Cell Biol. 7, 415–425 (2006).

    Article  CAS  Google Scholar 

  4. Baker, K.E. & Parker, R. Curr. Opin. Cell Biol. 16, 293–299 (2004).

    Article  CAS  Google Scholar 

  5. Muhlrad, D. & Parker, R. Nature 370, 578–581 (1994).

    Article  CAS  Google Scholar 

  6. Lejeune, F., Li, X. & Maquat, L.E. Mol. Cell 12, 675–687 (2003).

    Article  CAS  Google Scholar 

  7. Eberle, A.B., Lykke-Andersen, S., Muhlemann, O. & Jensen, T.H. Nat. Struct. Mol. Biol. 16, 49–55 (2009).

    Article  CAS  Google Scholar 

  8. Atkin, A.L. et al. J. Biol. Chem. 272, 22163–22172 (1997).

    Article  CAS  Google Scholar 

  9. Mangus, D.A. & Jacobson, A. Methods 17, 28–37 (1999).

    Article  CAS  Google Scholar 

  10. Sheth, U. & Parker, R. Cell 125, 1095–1109 (2006).

    Article  CAS  Google Scholar 

  11. Isken, O. et al. Cell 133, 314–327 (2008).

    Article  CAS  Google Scholar 

  12. Muhlrad, D. & Parker, R. Mol. Biol. Cell 10, 3971–3978 (1999).

    Article  CAS  Google Scholar 

  13. Hu, W., Sweet, T.J., Chamnongpol, S., Baker, K.E. & Coller, J. Nature 461, 225–229 (2009).

    Article  CAS  Google Scholar 

  14. Stalder, L. & Muhlemann, O. RNA 15, 1265–1273 (2009).

    Article  CAS  Google Scholar 

  15. Kshirsagar, M. & Parker, R. Genetics 166, 729–739 (2004).

    Article  CAS  Google Scholar 

  16. Decker, C.J., Teixeira, D. & Parker, R. J. Cell Biol. 179, 437–449 (2007).

    Article  CAS  Google Scholar 

  17. Rehwinkel, J., Behm-Ansmant, I., Gatfield, D. & Izaurralde, E. RNA 11, 1640–1647 (2005).

    Article  CAS  Google Scholar 

  18. Coller, J. & Parker, R. Cell 122, 875–886 (2005).

    Article  CAS  Google Scholar 

  19. Inada, T. et al. RNA 8, 948–958 (2002).

    Article  CAS  Google Scholar 

  20. Wang, Z., Jiao, X., Carr-Schmid, A. & Kiledjian, M. Proc. Natl. Acad. Sci. USA 99, 12663–12668 (2002).

    Article  CAS  Google Scholar 

Download references

Acknowledgements

The authors thank members of the Baker and Coller laboratories for critical evaluation of the manuscript. W.H. is supported by the American Heart Association. C.P. is supported by an undergraduate stipend provided by the US National Science Foundation. Funding was provided by the US National Institutes of Health (GM080465).

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  1. Center for RNA Molecular Biology, Case Western Reserve University, Cleveland, Ohio, USA

    Wenqian Hu, Christine Petzold, Jeff Coller & Kristian E Baker

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  1. Wenqian Hu
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  2. Christine Petzold
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  3. Jeff Coller
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  4. Kristian E Baker
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Contributions

W.H., J.C. and K.E.B. designed experimental strategies; W.H. and C.P. performed experimental analysis and interpreted data; J.C. and K.E.B. interpreted data and wrote the manuscript.

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Correspondence to Jeff Coller or Kristian E Baker.

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The authors declare no competing financial interests.

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Supplenentary Figures 1–4, Supplementary Table 1 and Supplementary Data (PDF 3446 kb)

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Hu, W., Petzold, C., Coller, J. et al. Nonsense-mediated mRNA decapping occurs on polyribosomes in Saccharomyces cerevisiae. Nat Struct Mol Biol 17, 244–247 (2010). https://doi.org/10.1038/nsmb.1734

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