Abstract
The NMR structure of budding yeast chaperone Chz1 complexed with histones H2A.Z-H2B has been determined. Chz1 forms a long irregular chain capped by two short α-helices, and uses both positively and negatively charged residues to stabilize the histone dimer. A molecular model that docks Chz1 onto the nucleosome has implications for its potential functions.
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References
De Koning, L., Corpet, A., Haber, J.E. & Almouzni, G. Nat. Struct. Mol. Biol. 14, 997–1007 (2007).
Mizuguchi, G. et al. Science 303, 343–348 (2003).
Luk, E. et al. Mol. Cell 25, 357–368 (2007).
Wu, W.H. et al. Nat. Struct. Mol. Biol. 12, 1064–1071 (2005).
Suto, R.K., Clarkson, M.J., Tremethick, D.J. & Luger, K. Nat. Struct. Biol. 7, 1121–1124 (2000).
English, C.M., Adkins, M.W., Carson, J.J., Churchill, M.E. & Tyler, J.K. Cell 127, 495–508 (2006).
Natsume, R. et al. Nature 446, 338–341 (2007).
Barbera, A.J. et al. Science 311, 856–861 (2006).
Luger, K., Mader, A.W., Richmond, R.K., Sargent, D.F. & Richmond, T.J. Nature 389, 251–260 (1997).
Hansen, D.F. et al. J. Am. Chem. Soc. 129, 11468–11479 (2007).
Acknowledgements
We thank A. Bax, C. Klee and M. Lichten for comments. This work was supported by the intramural program of the US National Cancer Institute (C.W. and Y.B.) and a grant from the Canadian Institutes of Health Research (L.E.K.). D.F.H. is the recipient of a postdoctoral fellowship from the Danish Agency for Science, Technology and Innovation.
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Zhou, Z., Feng, H., Hansen, D. et al. NMR structure of chaperone Chz1 complexed with histones H2A.Z-H2B. Nat Struct Mol Biol 15, 868–869 (2008). https://doi.org/10.1038/nsmb.1465
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DOI: https://doi.org/10.1038/nsmb.1465
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