The crystal structure of human replication and transcription cofactor PC4CTD reveals a dimer with two single-stranded (ss)DNA binding channels running in opposite directions to each other. This arrangement suggests a role in establishment or maintenance of melted DMA at promoters or origins of replication.
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References
Kaiser, K. & Meisterernst, M. Trends Biochem. Sci. 21, 342–345 (1996).
Ge, H. & Roeder, R.G. Cell 78, 513–523 (1994).
Kretzschmar, M., Kaiser, K., Lottspeich, F. & Meisterernst, M. Cell 78, 525–534 (1994).
Kaiser, K., Stelzer, G. & Meisterernst, M. EMBO J. 14, 3520–3527 (1995).
Ge, H., Zhao, Y., Chait, B.T. & Roeder, R.G. Natl. Acad Sci. USA 91, 12691–12695 (1994).
Nicholls, A., Sharp, K.A. & Honig, B. Proteins: Struct. Funct. Genet. 11, 281–296 (1991).
Knaus, R., Pollock, R. & Guarente, L. EMBO J. 15, 1933–1940 (1996).
Henry, N.L., Bushnell, D.A. & Kornberg, R.D. J. Biol. Chem. 271, 21842–21847 (1996).
Wallace, A.C., Laskowski, R.A. & Thornton, J.M. Prot. Eng. 8, 127–134 (1995).
Holm, L. & Sander, C. J. Mol. Biol. 233, 123–138 (1993).
Kleywegt, G.J. & Jones, T.A. In From first mask to final model, Proceedings of the CCP4 Study Weekend 1994 (eds Bailey S., Hubbard R. & Waller, D.) 59–66 (SERC Daresbury laboratory, Daresbury UK;1994).
Bochkarev, A., Pfuetzner, R.A., Edwards, A.M. & Frappier, L. Nature 385, 176–181 (1997).
Kong, X.-P., Onrust, R., O'Donnell, M. & Kuriyan, J. Cell 69, 425–437 (1992).
Krishna, T.S.R., Kong, X.-P., Gary, S., Burgers, P.M. & Kuriyan, J. Cell 79, 1233–1243 (1994).
Antson, A.A. et al. Nature 374, 693–700 (1995).
Lima, C.D., Wang, J.C. & Mondragon, A. Nature 367, 138–146 (1994).
Pan, Z.-Q., Ge, H., Amin, A.A. & Hurwitz, J. J. Biol. Chem. 271, 22111–22116 (1996).
Ravelli, R.B.G., Sweet, R.M., Skinner, J., Duisenberg, A.J.M. & Kroon, J. J. Appl. Crystallogr., in the press.
Otwinowski, Z. In Data Collection and Processing, Proceedings of the CCP4 Study Weekend 1993 (eds. Sawyer, L, Isaacs, N.W. & Bailey, S.) 55–62 (SERC Daresbury Laboratory, Daresbury UK (1993).
Collaborative Computational Project, Number 4, Acta Crystallogr. D50, 760–763 (1994).
Furey, W. & Swaminathan, S. Meth. Enz. in the press.
La Fortelle, E.de & Bricogne, G. Meth. Enz. in the press.
Abrahams, J.P. & Leslie, A.G.W. Acta Crystallogr. D52, 30–42 (1996).
Jones, T.A., Zou, J.Y., Cowan, S.W. & Kjelgaard, M. Acta Crystallogr. A47, 110–119 (1991).
Cowtan, K.D. & Main, P. Acta Crystallogr. D52, 43–48 (1996).
Brünger, A.T., Kuriyan, J. & Karplus, M. Science 235, 458–460 (1987).
Murshudov, G.N., Vagin, A.A. & Dodson, E.J. Acta Crystallogr. D53, 240–255 (1997).
Lamzin, V.S. & Wilson, K.S. Acta Crystallogr. D49, 129–147 (1993).
Kraulis, P.J. J. Appl. Crystallogr. 24, 946–950 (1991).
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Brandsen, J., Werten, S., van der Vliet, P. et al. C-terminal domain of transcription cofactor PC4 reveals dimeric ssDNA binding site. Nat Struct Mol Biol 4, 900–903 (1997). https://doi.org/10.1038/nsb1197-900
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DOI: https://doi.org/10.1038/nsb1197-900
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