Protein production: feeding the crystallographers and NMR spectroscopists

Article metrics

Abstract

Protein purification efforts for structural genomics will focus on automation for the readily-expressed proteins, and process development for the more difficult ones, such as membrane proteins. Thousands of proteins are expected to be produced in the next few years. The purified proteins will be valuable reagents for the entire research community.

Access options

Rent or Buy article

Get time limited or full article access on ReadCube.

from$8.99

All prices are NET prices.

References

  1. 1

    Kim, S.H. Nature Struct. Biol. 5, 643–645 (1998).

  2. 2

    Christendat, D. et al. Nature Struct. Biol. 7, 903– 909 (2000).

  3. 3

    Christendat, D. et al. Progr. Biophys. and Mol. Biol. in the press (2000).

  4. 4

    Hendrickson, W.A., Horton, J.R. & LeMaster, D.M. EMBO J. 9, 1665– 1672 (1990).

  5. 5

    Bax, A., Ikura, M., Kay, L.E., Barbato, G. & Spera, S. Ciba Found. Symp. 161, 108– 119 (1991).

  6. 6

    Eberstadt, M. et al. Nature 392, 941–945 (1998).

  7. 7

    Hansen, A.P. et al. Biochemistry 31, 12713– 12718 (1992).

  8. 8

    Kigawa, T. et al. FEBS Lett. 442, 15–19 (1999).

  9. 9

    Cohen, S.L., Ferre-D'Amare, A.R., Burley, S.K. & Chait, B.T. Protein Sci. 4, 1088–1099 (1995).

  10. 10

    Pfuetzner, R.A., Bochkarev, A., Frappier, L. & Edwards, A.M. J. Biol. Chem. 272, 430–434 (1997).

  11. 11

    Barwell, J.A. et al. J. Biol. Chem. 270, 20556– 20559 (1995).

  12. 12

    Bochkareva, E., Frappier, L., Edwards, A.M. & Bochkarev, A. J. Biol. Chem. 273, 3932–3936 (1998).

  13. 13

    Koth, C.M. et al. J. Biol. Chem. 275, 11174– 11180 (2000).

  14. 14

    Waldo, G.S., Standish, B.M., Berendzen, J. & Terwilliger, T.C. Nature Biotech. 17, 691–695 (1999).

  15. 15

    Maxwell, K.L., Mittermaier, A.K., Forman-Kay, J.D. & Davidson, A.R. Protein Sci. 8, 1908–1911 (1999).

Download references

Acknowledgements

The authors would like to acknowledge the research support of the Ontario government, the University of Toronto and the Ontario Cancer Institute. A.M.E. and C.H.A. are Scientists of the Canadian Institutes of Health Research (CIHR). J.F.G. is a Distinguished Scientist of the CIHR and a Foreign Investigator of the Howard Hughes Medical Institute. D.C. was supported by a Best Fellowship.

Author information

Correspondence to Aled M. Edwards.

Rights and permissions

Reprints and Permissions

About this article

Further reading