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Structure of the receptor binding fragment HC of tetanus neurotoxin

Nature Structural Biology volume 4, pages 788–792 (1997)Cite this article

The 2.7 Å structure of the tetanus neurotoxin receptor binding fragment HC reveals a jelly-roll domain and a β-trefoil domain. HC retains the unique transport properties of the holotoxin and is capable of eliciting a protective immunological response against the full length holotoxin.

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References

  1. Schiavo, G., Rossetto, O. & Montecucco, C. Seminars in Cell Biol. 5, 221–229 (1994).

  2. Lebeda, F.J. & Olson, M.A. Proteins: Struct. Funct. Genet. 20, 293–300 (1994).

    Article  CAS  Google Scholar 

  3. Minton, N.P. in Clostridial Neurotoxins: The molecular pathogenesis of tetanus and botulism (ed. Montecucco, C.) 161–194 (Springer, Berlin, Germany; 1995).

    Book  Google Scholar 

  4. Schiavo, G., et al. Nature (London) 359, 832–835 (1992).

    Article  CAS  Google Scholar 

  5. Schiavo, G. & Montecucco, C. in Bacterial Toxins: Tools in Cell Biology and Pharmacology (ed. Aktories, K.) 169–192 (Chapman & Hall, London, UK; 1997).

    Book  Google Scholar 

  6. Matteoli, M., et al. Proc. Natl. Acad. Sci. USA 93, 13310–13315 (1996).

    Article  CAS  Google Scholar 

  7. Halpern, J.L. & Neale, E.A. in Clostridial Neurotoxins: The molecular pathogenesis of tetanus and botulism (ed. Montecucco, C.) 221–241 (Springer, Berlin, Germany; 1995).

    Book  Google Scholar 

  8. Bizzini, B., Stoekel, K. & Schwab, M. J. Neurochem. 28, 529–542 (1977).

    Article  CAS  Google Scholar 

  9. Francis, J.W., Hosler, B.A., Brown, R.H., Jr. & Fishman, P.S. J. Biol. Chem. 270, 15434–15442 (1995).

    Article  CAS  Google Scholar 

  10. Montecucco, C. Trends Biochem. Sci. 11, 314–317 (1986).

    Article  CAS  Google Scholar 

  11. Bakry, N., Kamata, Y., Sorensen, R. & Simpson, L.L. J. Pharm. Exp. Therapeutics 258, 613–619 (1991).

    CAS  Google Scholar 

  12. Pierce, E.J., Davison, M.D., Parton, R.G., Habig, W.H. & Critchley, D.R. Biochem. J. 236, 845–852 (1986).

    Article  CAS  Google Scholar 

  13. Price, D.L., Griffen, J., Young, A., Peck, K. & Stocks, A. Science 188, 945–947 (1975).

    Article  CAS  Google Scholar 

  14. Montal, M.S., Blewitt, R., Tomich, J.M. & Montal, M. FEBS Lett. 313, 12–18 (1992).

    Article  CAS  Google Scholar 

  15. Sharon, N. Trends Biochem. Sci. 18, 221–226 (1993).

    Article  CAS  Google Scholar 

  16. Liao, D.-I., Kapadia, G., Ahmed, H., Vasta, G.R. & Herzberg, O. Proc. Natl. Acad. Sci. USA 91, 1428–1432 (1994).

    Article  CAS  Google Scholar 

  17. Murzin, A.G., Lesk, A.M. & Chothia, C. J. Mol. Biol. 223, 531–543 (1992).

    Article  CAS  Google Scholar 

  18. van Heyningen, W.E. Nature 249, 415–417 (1974).

    Article  CAS  Google Scholar 

  19. Halpern, J.L. & Loftus, A. J. Biol. Chem. 268, 11188–11192 (1993).

    CAS  PubMed  Google Scholar 

  20. Nishiki, T.-i., et al. J. Biol. Chem. 269, 10498–10503 (1994).

    CAS  PubMed  Google Scholar 

  21. Umland, T.C., Wingert, L., Swaminathan, S., Schmidt, J.J. & Sax, M. Acta Crystallogr. D, in the Press.

  22. Howard, A.J., Gilliland, G.L., Finzel, B.C. & Poulos, T.L. J. Appl. Crystallogr. 20, 383–387 (1987).

    Article  CAS  Google Scholar 

  23. Weissman, L. in Computational Crystallography (ed. Sayre, D.) 56–63 (Clarendon Press, Oxford, UK; 1982).

    Google Scholar 

  24. Derewenda, Z. & Helliwell, J.R. J. Appl. Crystallogr. 22, 123–137 (1989).

    Article  CAS  Google Scholar 

  25. Furey, W. & Swaminathan, S. in Macromolecular Crystallography (eds Carter, C. & Sweet, R.) (Academic Press, Orlando, Florida; 1997) in the press.

    Google Scholar 

  26. Jones, T.A., Zou, J.-Y., Cowan, S.W. & Kjeldgaard, M. Acta Crystallogr. sect. A 47, 110–119 (1991).

    Article  Google Scholar 

  27. Bruenger, A.T., Kuriyan, J. & Karplus, M. Science 235, 458–460 (1987).

    Article  CAS  Google Scholar 

  28. Kabsch, W. & Sander, C. Biopolymers 22, 2577–2637 (1983).

    Article  CAS  Google Scholar 

  29. Kraulis, P.J. J. Appl. Crystallogr. 24, 946–950 (1991).

    Article  Google Scholar 

  30. Carson, M.J. Appl. Crystallogr. 24, 958–961 (1991).

    Article  Google Scholar 

Download references

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Author notes

  1. Timothy C. Umland

    Present address: Building 5, Room 335, NIDDK, National Institutes of Health, Bethesda, Maryland, 20892, USA

  2. S. Swaminathan

    Present address: Biology Department, Building 463, Brookhaven National Laboratory, Upton, New York, 11973, USA

Authors and Affiliations

  1. Biocrystallography Laboratory, P.O. Box 12055, VA Medical Centre, Pittsburgh, Pennsylvania, 15240, USA

    Timothy C. Umland, Lavinia M. Wingert, S. Swaminathan, William F. Furey & Martin Sax

  2. Department of Crystallography, University of Pittsburgh, Pittsburgh, Pennsylvania, 15260, USA

    Lavinia M. Wingert, William F. Furey & Martin Sax

  3. Toxinology Division, U.S. Army Medical Research Institute of Infectious Diseases, Ft. Detrick, Frederick, Maryland, 21702, USA

    James J. Schmidt

Authors
  1. Timothy C. Umland
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  2. Lavinia M. Wingert
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  4. William F. Furey
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  5. James J. Schmidt
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  6. Martin Sax
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Umland, T., Wingert, L., Swaminathan, S. et al. Structure of the receptor binding fragment HC of tetanus neurotoxin. Nat Struct Mol Biol 4, 788–792 (1997). https://doi.org/10.1038/nsb1097-788

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