The three-dimensional structure of rice dwarf virus was determined to 6.8 Å resolution by single particle electron cryomicroscopy. By integrating the structural analysis with bioinformatics, the folds of the proteins in the double-shelled capsid were derived. In the outer shell protein, the uniquely orientated upper and lower domains are composed of similar secondary structure elements but have different relative orientations from that of bluetongue virus in the same Reoviridae family. Differences in both sequence and structure between these proteins may be important in defining virus–host interactions. The inner shell protein adopts a conformation similar to other members of Reoviridae, suggesting a common ancestor that has evolved to infect hosts ranging from plants to animals. Symmetry mismatch between the two shells results in nonequivalent, yet specific, interactions that contribute to the stability of this large macromolecular machine.
Access optionsAccess options
Subscribe to Journal
Get full journal access for 1 year
only $18.75 per issue
All prices are NET prices.
VAT will be added later in the checkout.
Rent or Buy article
Get time limited or full article access on ReadCube.
All prices are NET prices.
Gouet, P. et al. Cell 97, 481–490 (1999).
Lawton, J.A., Estes, M.K. & Prasad, B.V. Nature Struct. Biol. 4, 118–121 (1997).
Nibert, M.L., Schiff, L.A. & Field, B.N. in Fields Virology, Vol. 2 (eds. Fields, B.N. et al.) 1557–1596 (Lippincott-Raven, Philadelphia; 1996).
Lu, G. et al. J. Virol. 72, 8541–8549 (1998).
Zhu, Y. et al. J. Virol. 71, 8899–8901. (1997).
Wu, B. et al. Virology 271, 18–25. (2000).
Shao, C., Zhou, Z.H. & Lu, G. Science in China 44, 192–198 (2001).
Zhou, Z.H. et al. Science 288, 877–880 (2000).
Jiang, W., Baker, M.L., Ludtke, S.J. & Chiu, W. J. Mol. Biol. 308, 1033–1044 (2001).
Grimes, J.M. et al. Nature 395, 470–477 (1998).
Reinisch, K.M., Nibert, M.L. & Harrison, S.C. Nature 404, 960–967. (2000).
McGuffin, L.J., Bryson, K. & Jones, D.T. Bioinformatics 16, 404–405. (2000).
Rost, B., Sander, C. & Schneider, R. Comput. Appl. Biosci. 10, 53–60. (1994).
Baldi, P., Brunak, S., Frasconi, P., Soda, G. & Pollastri, G. Bioinformatics 15, 937–946. (1999).
Mizuguchi, K. & Go, N. Protein Eng. 8, 353–362. (1995).
Kleywegt, G.J. & Jones, T.A. Methods Enzymol. 277, 525–545 (1997).
Fisher, D. & Eisenberg, D. Protein Sci. 5, 947–955 (1996).
Grimes, J.M., Basak, A.K., Roy, P. & Stuart, D.I. Nature 373, 167–170 (1995).
Grimes, J.M. et al. Structure 5, 885–893 (1997).
Pesavento, J.B., Lawton, J.A., Estes, M.K. & Prasad, B.V. Proc. Natl. Acad. Sci. USA 98, 1381–1386. (2001).
Zhang, H. et al. J. Virol. 73, 1624–1629. (1999).
Fuller, S.D. Cell 48, 923–934 (1987).
Zhou, Z.H. et al. Nature Struct. Biol. 2, 1026–1030 (1995).
Zhou, Z.H. et al. Biophys. J. 74, 576–588 (1998).
Zhou, Z.H., Chen, D.H., Jakana, J., Rixon, F.J. & Chiu, W. J. Virol. 73, 3210–3218 (1999).
Crowther, R.A. Phil. Trans. R Soc. Lond. B Biol. Sci. 261, 221–230 (1971).
van Heel, M. Ultramicroscopy 21, 95–100 (1987).
Böttcher, B., Wynne, S.A. & Crowther, R.A. Nature 386, 88–91 (1997).
He, J., Schmid, M.F., Zhou, Z.H., Rixon, F. & Chiu, W. J. Mol. Biol. 309, 903–914 (2001).
Ludtke, S.J., Baldwin, P.R. & Chiu, W. J. Struct. Biol. 128, 82–97 (1999).
This research has been supported by grants from National Institutes of Health, the Robert Welch Foundation and the National Natural Science Foundation of China. Z.H.Z. is a Pew Scholar in the Biomedical Sciences and a Basil O'Connor Starter Scholar of the March of Dimes Foundation. M.L.B. was supported in part by Baylor Research Advocates for Student. We would like to thank B.V.V. Prasad, M.F. Schmid and M. Baker for their helpful comments on the manuscript. Supplementary animations and 3D VRML models are available at http://ncmi.bcm.tmc.edu/~wjiang/rdv/.
About this article
Cryo-EM reconstruction of the Cafeteria roenbergensis virus capsid suggests novel assembly pathway for giant viruses
Scientific Reports (2017)
Nature Communications (2017)
Journal of Structural Biology (2016)
Journal of Virology (2015)
PLOS ONE (2015)