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Structure of the Ras-binding domain of RalGEF and implications for Ras binding and signalling

Nature Structural Biology volume 4, pages 694699 (1997) | Download Citation

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The solution structure of the Ras-binding domain (RBD) of Ral guanine-nucleotide exchange factor RalGEF was solved by NMR spectroscopy. The overall structure is similar to that of Raf-RBD, another effector of Ras, although the sequence identity is only 13%. 1SN chemical shifts changes in the complex of RalGEF-RBD with Ras indicate an interaction similar to the intermolecular β-sheet observed for the complex between Ras and Raf-RBD.

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Affiliations

  1. Max-Planck-Institut für medizinische Forschung, Abteilung Biophysik, Jahnstraβle 29,69120 Heidelberg, Germany.

    • Matthias Geyer
    •  & Hans Robert Kalbitzer
  2. Max-Planck-Institut für molekulare Physiologie, Abteilung Strukturelle Biologie, Rheinlanddamm 201,44139 Dortmund, Germany.

    • Christian Herrmann
    • , Sabine Wohlgemuth
    •  & Alfred Wittinghofer
  3. kalbitz&mpimf-heidelberg.mpg.de

    • Hans Robert Kalbitzer

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DOI

https://doi.org/10.1038/nsb0997-694

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