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Confirmation of the arginine-finger hypothesis for the GAP-stimulated GTP-hydrolysis reaction of Ras

Nature Structural Biology volume 4, pages 686689 (1997) | Download Citation

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RasGAPs supply a catalytic residue, termed the arginine finger, into the active site of Ras thereby stabilizing the transition state of the GTPase reaction and increasing the reaction rate by more than one thousand-fold, in good agreement with the structure of the RasṁRasGAP complex.

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References

  1. 1.

    & Cell. Signal 6, 247–267 (1994).

  2. 2.

    & Annu. Rev. Biochem. 62, 851–891 (1993).

  3. 3.

    et al. Nature Struct. Biol. 2, 36–44 (1995).

  4. 4.

    , , , & Biochemistry 35, 14225–14231 (1996).

  5. 5.

    et al. Science 242, 1697–1700 (1988).

  6. 6.

    , , , & Proc. Natl. Acad. Sci. USA. 85, 5026–5030 (1988).

  7. 7.

    & Nature 351, 576–579 (1991).

  8. 8.

    et al. Mol. Cell. Biol. 12, 2050–2056 (1992).

  9. 9.

    , , , & J. Biol. Chem. 268, 27012–27019 (1993).

  10. 10.

    & Science 238, 542–545 (1987).

  11. 11.

    et al. Nature 335, 90–93 (1988).

  12. 12.

    et al. Cell 62, 599–608 (1990).

  13. 13.

    et al. Cell 63, 835–841 (1991).

  14. 14.

    et al. Cell 63, 851–859 (1990).

  15. 15.

    et al. Cell 63, 843–849 (1991).

  16. 16.

    , & In GTPases in Biology I, Handbook of Experimental Pharmacology, Vol. 108/1 (eds Dickey, B.F., & Birnbauer, L.) 195–212 (1993).

  17. 17.

    , & Proc. Natl. Acad. Sci. USA 93, 8160–8166 (1996).

  18. 18.

    , & FEBS Let. 410, 63–67 (1997).

  19. 19.

    , , & J. Biol. Chem. 263, 19718–19722 (1988).

  20. 20.

    , & Biochemistry 32, 7451–7459 (1993).

  21. 21.

    , , & Biochemistry 34, 15592–15598 (1995).

  22. 22.

    , , & Biochemistry 30, 11181–11185 (1991).

  23. 23.

    , , & Science 273, 115–117 (1996).

  24. 24.

    , , & Biochemistry, 36, 4535–4541 (1997).

  25. 25.

    & Biochemistry 36, 9290–9296 (1997).

  26. 26.

    , , & Proc. Natl. Acad. Sci 94, 3579–3583 (1996).

  27. 27.

    , , , & Nature 384, 591–596 (1996).

  28. 28.

    , , , , , & Science 277, 333–338 (1997).

  29. 29.

    , , , & J. Biol. Chem. 271, 16409–16415 (1996).

  30. 30.

    et al. Oncogene 8, 761–769 (1993).

  31. 31.

    & Chem. Biol. 2, 729–739 (1995).

  32. 32.

    et al. Science 265, 1405–1412 (1994).

  33. 33.

    , , , & Nature 372, 276–279 (1994).

  34. 34.

    , & Cell 44, 167–176 (1986).

  35. 35.

    , , , & Nature 312, 71–75 (1984).

  36. 36.

    et al. J. Biol. Chem. 266, 14163–14166 (1991).

  37. 37.

    , , , & J. Biol. Chem. 268, 10914–10919 (1993).

  38. 38.

    , , & J. Biol. Chem. 271, 15322–15329. (1996).

  39. 39.

    , , & Nucleic Acids Res. 22, 2587–2591 (1994).

  40. 40.

    et al. Bio/Technology 10, 900–904 (1992).

  41. 41.

    et al. EMBO J. 5, 1351–1358 (1986).

  42. 42.

    et al. Biochemistry 29, 6059–6065 (1990).

  43. 43.

    J. appl. Crystallogr. 24, 946–950 (1991).

  44. 44.

    & Raser3D version 2.0. Acta Crystallogr. D50, 869–873 (1994).

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Author information

Affiliations

  1. Max-Planck-Institut für molekulare Physiologie, Rheinlanddamm 201,44139 Dortmund, Germany.

    • Mohammad Reza Ahmadian
    • , Patricia Stege
    • , Klaus Scheffzek
    •  & Alfred Wittinghofer
  2. Alfred.Wittinghofer@mpi-dortmund.mpg.de

    • Alfred Wittinghofer

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DOI

https://doi.org/10.1038/nsb0997-686

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