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Trapping and visualization of a covalent enzyme–phosphate intermediate

Nature Structural Biology volume 4, pages 618–622 (1997)Cite this article

Using a mutant version of E. coli alkaline phosphatase, we succeeded in trapping and determining the structure of the phospho-enzyme intermediate. The X-ray structure also revealed the catalytic water molecule, bound to one of the active site zinc ions, positioned ideally for the apical attack necessary for the hydrolysis of the intermediate.

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Author notes

  1. Jennifer E. Murphy

    Present address: Jennifer M. Schneiders, Yale University School of Medicine, Department of Molecular Biophysics and Biochemistry, P.O. Box 208024, 333 Cedar Street, New Haven, Connecticut, 06520-8024, USA

Authors and Affiliations

  1. Department of Chemistry, Merkert Chemistry Center, Boston College, Chestnut Hill, Massachusetts, 02167, USA

    Boguslaw Stec, Lan Ma & Evan R. Kantrowitz

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  1. Jennifer E. Murphy
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  2. Boguslaw Stec
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Murphy, J., Stec, B., Ma, L. et al. Trapping and visualization of a covalent enzyme–phosphate intermediate. Nat Struct Mol Biol 4, 618–622 (1997). https://doi.org/10.1038/nsb0897-618

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