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Structure of the DNA binding domain of E. coli SSB bound to ssDNA

Nature Structural Biology volume 7, pages 648–652 (2000)Cite this article

Abstract

The structure of the homotetrameric DNA binding domain of the single stranded DNA binding protein from Escherichia coli (Eco SSB) bound to two 35-mer single stranded DNAs was determined to a resolution of 2.8 Å. This structure describes the vast network of interactions that results in the extensive wrapping of single stranded DNA around the SSB tetramer and suggests a structural basis for its various binding modes.

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Figure 1: Structure of the SSBc–ssDNA complex.
Figure 2: Schematic diagrams of protein–ssDNA interactions for the 28-mer ssDNA.
Figure 3: Conformational variability within the SSBc–ssDNA complex.
Figure 4: Models for the (SSB)65 and (SSB)35 ssDNA binding modes.

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Acknowledgements

G.W. and T.M.L were supported in this work by the NIH. We gratefully acknowledge T. Ho for synthesis and purification of oligodeoxynucleotides, S. Korolev and K. Fütterer for help during data collection, and the staff of beamline BL9.1 of SSRL, beamline X4A of NSLS, and beamline 19ID of APS.

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  1. Srinivasan Raghunathan

    Present address: The University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Boulevard, Dallas, Texas, 75235-9050

Authors and Affiliations

  1. Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, Campus Box 8231, 660 S. Euclid Ave., Saint Louis, 63110, Missouri, USA

    Srinivasan Raghunathan, Alexander G. Kozlov, Timothy M. Lohman & Gabriel Waksman

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  1. Srinivasan Raghunathan
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  2. Alexander G. Kozlov
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  3. Timothy M. Lohman
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  4. Gabriel Waksman
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Correspondence to Timothy M. Lohman or Gabriel Waksman.

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Raghunathan, S., Kozlov, A., Lohman, T. et al. Structure of the DNA binding domain of E. coli SSB bound to ssDNA. Nat Struct Mol Biol 7, 648–652 (2000). https://doi.org/10.1038/77943

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