Structure of the negative regulatory domain of p53 bound to S100B(ββ)

Abstract

A Ca2+ dependent conformational change in dimeric S100B(ββ) is required for it to bind p53 and inhibit phosphorylation of this tumor suppressor in its C-terminal negative regulatory domain. A peptide derived from this region of p53 (residues 367–388) was found to have no regular structure in its native form by NMR spectroscopy, but becomes helical when bound to Ca2+ loaded S100B(ββ). The three-dimensional structure of this complex reveals several favorable hydrophobic and electrostatic interactions between S100B(ββ) and the p53 peptide in the binding pocket, where S100B(ββ) sterically blocks sites of phosphorylation and acetylation on p53 that are important for transcription activation.

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Figure 1: NMR data from the negative regulatory domain of p53 (unlabeled) bound to 13C,15N labeled S100B(ββ).
Figure 2: Comparison of S100B(ββ) structures in the apo, Ca2+ bound and p53 peptide bound states.
Figure 3: Positioning of side chain residues in the p53 peptide-S100B(ββ) complex.

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Acknowledgements

This work was supported by grants from the National Institutes of Health (to D.J.W.), the American Cancer Society (to D.J.W.), and SRIS/DRIF funding from The University of Maryland School of Medicine and the State of Maryland (to D.J.W.).

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Correspondence to David J. Weber.

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Rust, R., Baldisseri, D. & Weber, D. Structure of the negative regulatory domain of p53 bound to S100B(ββ) . Nat Struct Mol Biol 7, 570–574 (2000). https://doi.org/10.1038/76797

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