Structures of two short-lived intermediates during the photocycle of the photoactive yellow protein (PYP) have been determined using X-ray crystallography. These structures demonstrate the power of cryo-trapping and the Laue method in trapping transient intermediates.
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Farber, G. Sensing photons. Nat Struct Mol Biol 5, 415–417 (1998). https://doi.org/10.1038/nsb0698-415
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DOI: https://doi.org/10.1038/nsb0698-415