Paramagnetic relaxation measurements show that reduction of the cytochrome P450 BM3–substrate complex is accompanied by a structural change leading to a 6 Å movement of the substrate into the correct position and orientation for hydroxylation.
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Modi, S., Sutcliffe, M., Primrose, W. et al. The catalytic mechanism of cytochrome P450 BM3 involves a 6 Å movement of the bound substrate on reduction. Nat Struct Mol Biol 3, 414–417 (1996). https://doi.org/10.1038/nsb0596-414
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DOI: https://doi.org/10.1038/nsb0596-414
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