Abstract
Coordinated variations in the sequence of the Rev–binding element of HIV–1, identified by in vitro genetic selections, have been used as distance and conformational constraints for molecular modelling. Three–dimensional models of the wild–type Rev–binding element and several, evolved RNA ligands (aptamers) have been constructed. These models demonstrate that non–Watson–Crick pairings open the major groove allowing access of an α–helical peptide from Rev, and explain why some selected RNA sequences can bind Rev more tightly than others.
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Leclerc, F., Cedergren, R. & Ellington, A. A three–dimensional model of the Rev–binding element of HIV–1 derived from analyses of aptamers. Nat Struct Mol Biol 1, 293–300 (1994). https://doi.org/10.1038/nsb0594-293
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DOI: https://doi.org/10.1038/nsb0594-293
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