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Unraveling the means to the end in ATP-dependent proteases

In the ATP-dependent proteases, separate internal chambers function in substrate unfolding and degradation. These chambers are linked by narrow channels, requiring a protein to unfold completely in order to pass between them. New work suggests that substrates are unraveled from one end first and are translocated vectorially into the proteolytic core.

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Figure 1: Structures of HslVU, an ATP-dependent protease, and the DHFR substrates.
Figure 2: A possible pathway for DHFR CP Pro 25 vectorial unfolding beginning at its new N-terminus.

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Correspondence to Mark Hochstrasser.

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Hochstrasser, M., Wang, J. Unraveling the means to the end in ATP-dependent proteases. Nat Struct Mol Biol 8, 294–296 (2001). https://doi.org/10.1038/86153

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