In the ATP-dependent proteases, separate internal chambers function in substrate unfolding and degradation. These chambers are linked by narrow channels, requiring a protein to unfold completely in order to pass between them. New work suggests that substrates are unraveled from one end first and are translocated vectorially into the proteolytic core.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 12 print issues and online access
$189.00 per year
only $15.75 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
References
Horwich, A.L., Weber-Ban, E.U. & Finley, D. Proc. Natl. Acad. Sci. USA 96, 11033–11040 (1999).
Lee, C., Schwartz, M.P., Prakash, S., Iwakura, M. & Matouschek, A. Mol. Cell in the press (2001).
Hochstrasser, M. Annu. Rev. Genet. 30, 405–439 (1996).
Wickner, S., Maurizi, M.R. & Gottesman, S. Science 286, 1888–1893. (1999).
Weber-Ban, E.U., Reid, B.G., Miranker, A.D. & Horwich, A.L. Nature 401, 90–93 (1999).
Kim, Y.I., Burton, R.E., Burton, B.M., Sauer, R.T. & Baker, T.A. Mol. Cell 5, 639–648. (2000).
Hoskins, J.R., Singh, S.K., Maurizi, M.R. & Wickner, S. Proc. Natl. Acad. Sci. USA 97, 8892–8897. (2000).
Singh, S.K., Grimaud, R., Hoskins, J.R., Wickner, S. & Maurizi, M.R. Proc. Natl. Acad. Sci. USA 97, 8898–8903. (2000).
Shtilerman, M., Lorimer, G.H. & Englander, S.W. Science 284, 822–825. (1999).
Iwakura, M., Nakamura, T., Yamane, C. & Maki, K. Nature Struct. Biol. 7, 580–585. (2000).
Wang, J. et al. Structure Fold. Des. 9, 177–184 (2001).
Reid, B.G., Fenton, W.A., Horwich, A.L. & Weber-Ban, E.U. Proc. Natl. Acad. Sci. USA, 98, in the press (2001).
Gonzalez, M., Rasulova, F., Maurizi, M.R. & Woodgate, R. EMBO J. 19, 5251–5258. (2000).
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Hochstrasser, M., Wang, J. Unraveling the means to the end in ATP-dependent proteases. Nat Struct Mol Biol 8, 294–296 (2001). https://doi.org/10.1038/86153
Issue Date:
DOI: https://doi.org/10.1038/86153
This article is cited by
-
Order of the Proteasomal ATPases and Eukaryotic Proteasome Assembly
Cell Biochemistry and Biophysics (2011)
-
The mechanical stability of ubiquitin is linkage dependent
Nature Structural & Molecular Biology (2003)