The tide of the blood coagulation cascade turns by a switch in thrombin specificity. Two new structures reveal how thrombomodulin's EGF domains flip the switch by blocking old substrates and docking new ones.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 12 print issues and online access
$189.00 per year
only $15.75 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
References
Wood, M.J., Sampoli Benitez, B. A. & Komives, E. A. Nature Struct. Biol. 7, 200–204 (2000).
Fuentes-Prior, P. et al. Nature in the press (2000).
Ferry, J.D. & Morrison, P.R. J. Am. Chem. Soc. 69, 388–399 (1947).
Kisiel, W., Ericsson, L.H. & Davie, E.W. Biochemistry 15, 4893–4900 (1976).
Bajzar, L., Manuel, R. & Nesheim, M. E. J. Biol. Chem. 270, 14477–14484 (1995).
Esmon, C.T. & Owen, W.G. Proc. Natl. Acad. Sci. USA 78, 2249–2252 (1981).
Esmon, C.T. FASEB J. 9, 946–955 (1995).
White C.E. et al. Proc. Natl. Acad. Sci. USA 93, 10177–10182 (1996).
White, C.E. et al. Protein Eng. 8, 1177–1187 (1995).
Sampoli Benitez, B.A., Hunter, M. J., Meininger, D. P. & Komives, E.A. J. Mol. Biol. 273, 913–926 (1997).
Light, D.R. et al. Eur J. Biochem. 262, 522–533 (1999).
Mathews I.I., Padmanabhan, K.P., Tulinsky, A. & Sadler, J.E. Biochemistry 33, 13547–13552 (1994)
Mather, T. et al. EMBO J. 15, 6822–6831 (1996).
Zushi, M. J. Biol. Chem. 266, 19886–19889 (1991).
Knobe, K.E. et al. Proteins 35, 218–234 (1999).
Nesheim, M. et al. Thromb. Haemost. 78, 386–391 (1997).
Fareed, J. et al. Semin. Hematol. 36, 42–56 (1999).
Acknowledgements
We thank P. Fuentes-Prior and M.J. Wood for providing atomic coordinates, and J. Mamay for helping with figure preparation.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Overduin, M., de Beer, T. The plot thickens: How thrombin modulates blood clotting. Nat Struct Mol Biol 7, 267–269 (2000). https://doi.org/10.1038/74015
Issue Date:
DOI: https://doi.org/10.1038/74015
