Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Correspondence
  • Published:

Crystal structure of phytase from Aspergillus ficuum at 2.5 Å resolution

Phytase is a high molecular weight acid phosphatase. The structure has an α/β-domain similar to that of rat acid phosphatase and an α-domain with a new fold.

This is a preview of subscription content, access via your institution

Access options

Buy this article

Prices may be subject to local taxes which are calculated during checkout

References

  1. Vincent, J.B., Crowder, M.W. & Averill, B.A. Trends Biochem. Sci. 17, 105–110 (1992).

    Article  CAS  Google Scholar 

  2. Ullah, A.H.J. & Gibson, D.M. Prep. Biochem. 17, 63–91 (1987).

    CAS  PubMed  Google Scholar 

  3. Ullah, A.H.J. Prep. Biochem. 18, 459–471 (1988).

    CAS  PubMed  Google Scholar 

  4. Ullah, A.H.J., & Phillipy, B.Q. J. Agric. Food Chem. 42, 423–425 (1994).

    Article  CAS  Google Scholar 

  5. Van Etten, R.L. Ann. NY Acad. Sci. 390, 27–51 (1982).

    Article  CAS  Google Scholar 

  6. Schneider, G., Lindqvist, Y., & Vihko, P. EMBO J. 12, 2609–2615 (1993).

    Article  CAS  Google Scholar 

  7. Copley, R.R., & Barton, G.J. J. Mol. Biol. 242, 321–329 (1994).

    CAS  PubMed  Google Scholar 

  8. Ullah, A.H.J., & Dischinger, H.C. Jr. Ann. N.Y. Acad. Sci. 672, 45–51 (1982).

    Article  Google Scholar 

  9. Van Etten, R.L., Davidson, R., Stevis, P., MacArthur, H., & Moore, D. J. Biol. Chem. 266, 2313–2319 (1991).

    CAS  PubMed  Google Scholar 

  10. Diederichs, K. Proteins 23, 187–195 (1995).

    Article  CAS  Google Scholar 

  11. Bernstein, F.C., et al. J. Mol. Biol. 111, 535–542 (1977).

    Article  Google Scholar 

  12. Winn, S.I., Watson, H.C., Harkins, R.N. & Fothergill, L.A. Philos. Trans. R. Soc. Lond., B, 293, 121–130 (1981).

    Article  CAS  Google Scholar 

  13. Breiter, D.R. et al. Biochemistry 30, 603–608 (1991).

    Article  CAS  Google Scholar 

  14. Ostanin, K., et al. J. Biol. Chem. 267, 22830–22836 (1992).

    CAS  PubMed  Google Scholar 

  15. Gerber, P.R., & Müller, K. J. Computer-Aided Mol. Design, 9, 251–268 (1995).

    Article  CAS  Google Scholar 

  16. Nicholls, A. & Honig, B. “GRASP: Graphical representation and analysis of surface properties.” Columbia University, New York, (1992).

  17. Kabsch, W. J. Appl. Crystallogr. 21, 916–924 (1988).

    Article  CAS  Google Scholar 

  18. Collaborative Computational Project, Number 4. Acta Crystallogr. D50, 760–763 (1994).

  19. Jones, T.A., Zou, J.-Y., Cowan, S.W., & Kjeldgaard, M. Acta Crystallogr. A47, 110–119 (1991).

    Article  CAS  Google Scholar 

  20. Cowtan, K.D., & Main, P. Acta Crystallor. D49, 148–157 (1993).

    CAS  Google Scholar 

  21. Jones, T.A., & Thirup, S. EMBO J. 5, 819–822 (1986).

    Article  CAS  Google Scholar 

  22. Altschul, S.F., Grish, W., Miller, W., Myers, E.W., & Lipman, D.J. J. Mol. Biol. 215, 403–410 (1990).

    Article  CAS  Google Scholar 

  23. Bairoch, A., & Boeckmann, B. Nucl. Acids. Res. 19, 2247–2250 (1991).

    Article  CAS  Google Scholar 

  24. Bairoch, A., Bucher, P. & Hofmann, K. Nucl. Acids. Res. 24, 189–196 (1996).

    Article  CAS  Google Scholar 

  25. Brünger, A.T., Kuriyan, J., & Karplus, M. Science 235, 458–460 (1987).

    Article  Google Scholar 

  26. Brünger, A.T. X-PLOR Version 3.1–A System for X-ray Crystallography and NMR. Yale University Press, New Haven & London (1992).

    Google Scholar 

  27. Kostrewa, D. & Winkler, F.K. Biochemistry 34, 683–696 (1995).

    Article  CAS  Google Scholar 

  28. Matthews, B.W. J. Mol. Biol. 33, 491–497 (1968).

    Article  CAS  Google Scholar 

  29. Jiang, J.-S. & Brünger, A.T. J. Mol. Biol. 243, 100–115 (1994).

    Article  CAS  Google Scholar 

  30. Read, R.J. Acta Crystallogr. A42, 140–149 (1986).

    Article  CAS  Google Scholar 

  31. Engh, R.A., & Huber, R. Acta Crystallogr. A47, 392–400 (1991).

    Article  CAS  Google Scholar 

  32. Brünger, A.T. Nature 355, 472–475 (1992).

    Article  Google Scholar 

  33. Hodel, A., Kirn, S.-H., & Brünger, A.T. Acta Crystallogr. A48, 851–858 (1992).

    Article  CAS  Google Scholar 

  34. Kleywegt, G.J., & Jones, T.A. Acta Crystallogr. D52, 829–832 (1996).

    CAS  Google Scholar 

  35. Morris, A.L., MacArthur, M.W., Hutchinson, E.G., & Thornton, J.M. Proteins 12, 345–364 (1992).

    Article  CAS  Google Scholar 

  36. Kraulis, P.J. J. Appl. Crystallogr. 24, 946–950 (1991).

    Article  Google Scholar 

  37. Merritt, E.A., & Murphy, M.E.P. Acta Crystallogr. D50, 869–873 (1994).

    CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kostrewa, D., Grüninger-Leitch, F., D′Arcy, A. et al. Crystal structure of phytase from Aspergillus ficuum at 2.5 Å resolution. Nat Struct Mol Biol 4, 185–190 (1997). https://doi.org/10.1038/nsb0397-185

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1038/nsb0397-185

This article is cited by

Search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing